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Dexmedetomidine Protects Cardiomyocytes against HypoxiaReoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling
المؤلفون المشاركون
Gao, Jin-meng
Meng, Xiao-wen
Zhang, Juan
Chen, Wei-rong
Xia, Fan
Peng, Ke
Ji, Fu-hai
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-22
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Objective.
We previously reported that dexmedetomidine (DEX) offers cardioprotection against ischemia/reperfusion injury in rats.
Here, we evaluated the role of toll-like receptors 4- (TLR4-) myeloid differentiation primary response 88- (MyD88-) nuclear factor-kappa B (NF-κB) signaling in DEX-mediated protection of cardiomyocytes using in vitro models of hypoxia/reoxygenation (H/R).
Methods.
The experiments were carried out in H9C2 cells and in primary neonatal rat cardiomyocytes.
Cells pretreated with vehicle or DEX were exposed to hypoxia for 1 h followed by reoxygenation for 12 h.
We analyzed cell viability and lactate dehydrogenase (LDH) activity and measured tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β mRNA levels, TLR4, MyD88, and nuclear NF-κB p65 protein expression and NF-κB p65 nuclear localization.
TLR4 knock-down by TLR4 siRNA transfection and overexpression by TLR4 DNA transfection were used to further confirm our findings.
Results.
DEX protected against H/R-induced cell damage and inflammation, as evidenced by increased cell survival rates, decreased LDH activity, and decreased TNF-α, IL-6, and IL-1β mRNA levels, as well as TLR4 and NF-κB protein expression.
TLR4 knock-down partially prevented cell damage following H/R injury, while overexpression of TLR4 abolished the DEX-mediated protective effects.
Conclusions.
DEX pretreatment protects rat cardiomyocytes against H/R injury.
This effect is partly mediated by TLR4 suppression via TLR4-MyD88-NF-κB signaling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gao, Jin-meng& Meng, Xiao-wen& Zhang, Juan& Chen, Wei-rong& Xia, Fan& Peng, Ke…[et al.]. 2017. Dexmedetomidine Protects Cardiomyocytes against HypoxiaReoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1134110
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gao, Jin-meng…[et al.]. Dexmedetomidine Protects Cardiomyocytes against HypoxiaReoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1134110
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gao, Jin-meng& Meng, Xiao-wen& Zhang, Juan& Chen, Wei-rong& Xia, Fan& Peng, Ke…[et al.]. Dexmedetomidine Protects Cardiomyocytes against HypoxiaReoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1134110
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134110
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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