Dexmedetomidine Protects Cardiomyocytes against HypoxiaReoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling
المؤلفون المشاركون
Gao, Jin-meng
Meng, Xiao-wen
Zhang, Juan
Chen, Wei-rong
Xia, Fan
Peng, Ke
Ji, Fu-hai
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-22
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Objective.
We previously reported that dexmedetomidine (DEX) offers cardioprotection against ischemia/reperfusion injury in rats.
Here, we evaluated the role of toll-like receptors 4- (TLR4-) myeloid differentiation primary response 88- (MyD88-) nuclear factor-kappa B (NF-κB) signaling in DEX-mediated protection of cardiomyocytes using in vitro models of hypoxia/reoxygenation (H/R).
Methods.
The experiments were carried out in H9C2 cells and in primary neonatal rat cardiomyocytes.
Cells pretreated with vehicle or DEX were exposed to hypoxia for 1 h followed by reoxygenation for 12 h.
We analyzed cell viability and lactate dehydrogenase (LDH) activity and measured tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β mRNA levels, TLR4, MyD88, and nuclear NF-κB p65 protein expression and NF-κB p65 nuclear localization.
TLR4 knock-down by TLR4 siRNA transfection and overexpression by TLR4 DNA transfection were used to further confirm our findings.
Results.
DEX protected against H/R-induced cell damage and inflammation, as evidenced by increased cell survival rates, decreased LDH activity, and decreased TNF-α, IL-6, and IL-1β mRNA levels, as well as TLR4 and NF-κB protein expression.
TLR4 knock-down partially prevented cell damage following H/R injury, while overexpression of TLR4 abolished the DEX-mediated protective effects.
Conclusions.
DEX pretreatment protects rat cardiomyocytes against H/R injury.
This effect is partly mediated by TLR4 suppression via TLR4-MyD88-NF-κB signaling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gao, Jin-meng& Meng, Xiao-wen& Zhang, Juan& Chen, Wei-rong& Xia, Fan& Peng, Ke…[et al.]. 2017. Dexmedetomidine Protects Cardiomyocytes against HypoxiaReoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1134110
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gao, Jin-meng…[et al.]. Dexmedetomidine Protects Cardiomyocytes against HypoxiaReoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1134110
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gao, Jin-meng& Meng, Xiao-wen& Zhang, Juan& Chen, Wei-rong& Xia, Fan& Peng, Ke…[et al.]. Dexmedetomidine Protects Cardiomyocytes against HypoxiaReoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1134110
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134110
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر