Urethral Reconstruction Using Mesothelial Cell-Seeded Autogenous Granulation Tissue Tube: An Experimental Study in Male Rabbits
المؤلفون المشاركون
Jiang, Shiwei
Xu, Zhonghua
Zhao, Yuanyuan
Yan, Lei
Zhou, Zunlin
Gu, Gangli
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-02-28
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objective.
This study was to evaluate the utility of the compound graft for tubularized urethroplasty by seeding mesothelial cells onto autogenous granulation tissue.
Methods.
Silastic tubes were implanted subcutaneously in 18 male rabbits, of which nine underwent omentum biopsies simultaneously for in vitro expansion of mesothelial cells.
The granulation tissue covering the tubes was harvested 2 weeks after operation.
Mesothelial cells were seeded onto and cocultured with the tissue for 7 days.
A pendulous urethral segment of 1.5 cm was totally excised.
Urethroplasty was performed with mesothelial cell-seeded tissue tubes in an end-to-end fashion in nine rabbits and with unseeded grafts in others as controls.
Serial urethrograms were performed at 1, 2, and 6 months postoperatively.
Meanwhile, the neourethra was harvested and analyzed grossly and histologically.
Results.
Urethrograms showed cell-seeded grafts maintained wide at each time point, while strictures formation was found in unseeded grafts.
Histologically, layers of urothelium surrounded by increasingly organized smooth muscles were observed in seeded grafts.
In contrast, myofibroblasts accumulation and extensive scarring occurred in unseeded grafts.
Conclusions.
Mesothelial cell-seeded granulation tissue tube can be successfully used for tubularized urethroplasty in male rabbits.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Shiwei& Xu, Zhonghua& Zhao, Yuanyuan& Yan, Lei& Zhou, Zunlin& Gu, Gangli. 2017. Urethral Reconstruction Using Mesothelial Cell-Seeded Autogenous Granulation Tissue Tube: An Experimental Study in Male Rabbits. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1134255
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Shiwei…[et al.]. Urethral Reconstruction Using Mesothelial Cell-Seeded Autogenous Granulation Tissue Tube: An Experimental Study in Male Rabbits. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1134255
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Shiwei& Xu, Zhonghua& Zhao, Yuanyuan& Yan, Lei& Zhou, Zunlin& Gu, Gangli. Urethral Reconstruction Using Mesothelial Cell-Seeded Autogenous Granulation Tissue Tube: An Experimental Study in Male Rabbits. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1134255
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134255
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر