MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB
المؤلفون المشاركون
Wang, Yongxia
Wang, Beixi
Zhou, Hong
Zhang, Xiangnan
Qian, Xinlai
Cui, Jing
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-09
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development.
The abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers.
However, the function and the underlying mechanism of miR-384 in PTC progression remain largely unknown.
Methods.
Real-time PCR was conducted to detect miR-384 expression in 58 cases of PTC and their adjacent noncancerous tissues.
MTT, soft agar assay Transwell assay, and wound-healing assay were carried out to explore the biological function of miR-384 in PTC cell lines of BCPAP and K1.
Bioinformatics analysis, dual-luciferase reporter assay, western blot, and functional complementation analysis were conducted to explore the target gene of miR-384.
Moreover, Spearman’s correlation analysis was conducted to reveal the correlation between miR-384 and PRKACB mRNA in PTC.
Results.
The expression of miR-384 decreased obviously in PTC, especially in the tumors with lymph node metastasis or larger tumor size.
The ectopic upregulation of miR-384 significantly suppressed PTC progression, and the inhibition of miR-384 had the opposite effects.
Moreover, PRKACB gene was confirmed as the target of miR-384.
Conclusion.
The study suggests that miR-384 serves as a tumor suppressor in PTC progression by directly targeting the 3′-UTR of PRKACB gene.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yongxia& Wang, Beixi& Zhou, Hong& Zhang, Xiangnan& Qian, Xinlai& Cui, Jing. 2020. MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1134355
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yongxia…[et al.]. MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1134355
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yongxia& Wang, Beixi& Zhou, Hong& Zhang, Xiangnan& Qian, Xinlai& Cui, Jing. MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1134355
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134355
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر