Small RNA Sequencing Reveals Differentially Expressed miRNAs in Necrotizing Enterocolitis in Rats
المؤلفون المشاركون
Yu, Ren-qiang
Wang, Min
Jiang, Shan-yu
Zhang, Ying-hui
Zhou, Xiao-yu
Zhou, Qin
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-02
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Necrotizing enterocolitis (NEC) is the leading cause of death due to gastrointestinal disease in preterm infants.
The role of miRNAs in NEC is still unknown.
The objective of this study was to identify differentially expressed (DE) miRNAs in rats with NEC and analyze their possible roles.
In this study, a NEC rat model was established using Sprague-Dawley rat pups.
Small RNA sequencing was used to analyze the miRNA expression profiles in the NEC and control rats.
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were carried out to identify target mRNAs for the DE miRNAs and to explore their potential roles.
The DE miRNAs were verified by real-time quantitative PCR (RT-qPCR).
The status of intestinal injury and the elevated levels of inflammatory cytokines in the NEC group confirmed that the NEC model was successfully established.
The 16 miRNAs were found to be differentially expressed between the NEC group and the control group of rats.
Bioinformatics analysis indicated that the parental genes of the DE miRNAs were predominantly implicated in the phosphorylation, cell migration, and protein phosphorylation processes.
Moreover, the DE miRNAs were mainly found to be involved in the pathways of axon guidance, endocytosis, and focal adhesion, as well as in the Wnt signaling pathway, which is related to colitis.
The expression patterns of the candidate miRNAs (rno-miR-27a-5p and rno-miR-187-3p), as assessed by RT-qPCR, were in accordance with the expression patterns obtained by miRNA-sequencing.
The miRNA/mRNA/pathway network revealed that rno-miR-27a-5p and rno-miR-187-3p might be involved in NEC via the Wnt signaling pathway.
We found an altered miRNA expression pattern in rats with NEC.
We hypothesize that rno-miR-27a-5p and rno-miR-187-3p might mediate the NEC pathophysiological processes via the Wnt signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yu, Ren-qiang& Wang, Min& Jiang, Shan-yu& Zhang, Ying-hui& Zhou, Xiao-yu& Zhou, Qin. 2020. Small RNA Sequencing Reveals Differentially Expressed miRNAs in Necrotizing Enterocolitis in Rats. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134520
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yu, Ren-qiang…[et al.]. Small RNA Sequencing Reveals Differentially Expressed miRNAs in Necrotizing Enterocolitis in Rats. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1134520
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yu, Ren-qiang& Wang, Min& Jiang, Shan-yu& Zhang, Ying-hui& Zhou, Xiao-yu& Zhou, Qin. Small RNA Sequencing Reveals Differentially Expressed miRNAs in Necrotizing Enterocolitis in Rats. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134520
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134520
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر