Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
المؤلفون المشاركون
Jiao, Xiaoyang
Li, Yazhen
Chen, Jinghong
Qiu, Qiancheng
Fan, Zhiqiang
Yao, Fen
Shen, Wenjun
Sun, Jiayu
Yuan, Yumeng
Cai, Leshan
Xie, Yanxuan
Liu, Kaixi
Chen, Xiang
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-17
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Purpose.
Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC.
We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis.
Methods.
Cancerous and adjacent noncancerous tissues were obtained from CRC patients.
HPV antigen was measured by using the immunohistochemical (IHC) technique.
The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates.
The target genes were validated with the qPCR method.
Results.
15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues.
With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues.
Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs.
normal tissues, or adenoma vs.
normal tissue in TCGA, which was overlapped with our data.
In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue.
MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues.
Conclusion.
HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways.
Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qiu, Qiancheng& Li, Yazhen& Fan, Zhiqiang& Yao, Fen& Shen, Wenjun& Sun, Jiayu…[et al.]. 2020. Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma. BioMed Research International،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1134568
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qiu, Qiancheng…[et al.]. Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma. BioMed Research International No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1134568
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qiu, Qiancheng& Li, Yazhen& Fan, Zhiqiang& Yao, Fen& Shen, Wenjun& Sun, Jiayu…[et al.]. Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1134568
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134568
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر