Lanthanum Chloride Causes Neurotoxicity in Rats by Upregulating miR-124 Expression and Targeting PIK3CA to Regulate the PI3KAkt Signaling Pathway
المؤلفون المشاركون
Zheng, Linlin
Zhang, Jinhui
Yu, Shengjin
Ding, Zhe
Song, Heling
Wang, Yan
Li, Yicui
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-05
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Lanthanum (La) exposure can cause central nervous system (CNS) damage and dysfunction in children, seriously affecting intellectual development.
miR-124 plays an important role in the development of the nervous system.
We exposed rats to a La environment then observed the rats’ learning and memory damage and neurotoxicity and the relationship with miR-124.
Methods.
Rats were exposed to LaCl3 via drinking water.
The rats’ offspring were exposed to LaCl3 from their mother before weaning, then from La water for 28 days.
A Morris water maze was used to observe spatial memory capabilities.
H&E staining and TUNEL assays were used to observe pathological changes and apoptosis in the hippocampus.
miR-124 was detected by RT-qPCR, and its targeting was confirmed by luciferase assay.
The HT22 cell line was cultured with LaCl3 and treated with miR-124 mimics or inhibitors; then, expression of PI3K/Akt-related proteins was detected by western blot.
Results.
La exposure can lead to impaired learning and memory ability in offspring.
Offspring with La accumulations in the hippocampus showed severe damage, disordered cells, and increased neurocyte apoptosis.
In vitro, the postsynaptic density protein 95 was downregulated under La exposure and apoptosis increased.
This effect of La can be attenuated by miR-124 inhibitors and enhanced by miR-124 mimics.
LaCl3 exposure increased miR-124 expression and targeting on PIK3CA, downregulating PI3K, p-Akt, and p-NF-κB p65.
Conclusion.
La causes neurotoxicity by upregulating miR-124 expression and targeting PIK3CA through the PI3K/Akt signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zheng, Linlin& Zhang, Jinhui& Yu, Shengjin& Ding, Zhe& Song, Heling& Wang, Yan…[et al.]. 2020. Lanthanum Chloride Causes Neurotoxicity in Rats by Upregulating miR-124 Expression and Targeting PIK3CA to Regulate the PI3KAkt Signaling Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1134575
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zheng, Linlin…[et al.]. Lanthanum Chloride Causes Neurotoxicity in Rats by Upregulating miR-124 Expression and Targeting PIK3CA to Regulate the PI3KAkt Signaling Pathway. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1134575
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zheng, Linlin& Zhang, Jinhui& Yu, Shengjin& Ding, Zhe& Song, Heling& Wang, Yan…[et al.]. Lanthanum Chloride Causes Neurotoxicity in Rats by Upregulating miR-124 Expression and Targeting PIK3CA to Regulate the PI3KAkt Signaling Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1134575
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134575
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر