Minocycline Induces Autophagy and Inhibits Cell Proliferation in LPS-Stimulated THP-1 Cells
المؤلفون المشاركون
Sun, Jian
Shigemi, Hiroko
Cao, Miaoyin
Qin, E.
Tang, Jixian
Shen, Juxin
Iwasaki, Hiromichi
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-20
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Excessive activation and proliferation of inflammatory cell and uncontrolled release of cytokines and chemokines, also known as cytokine storm, is considered to be the main cause of sepsis.
Accumulating evidence has indicated that autophagy may play an important role in regulating immune response and controlling excessive inflammation.
Recent studies have showed that minocycline has immunomodulatory effects on cytokine and chemokine production.
It has also been reported that minocycline can induce autophagy, suggesting that autophagy may be involved in the process of minocycline regulating inflammation and immune response.
However, the precise mechanism is unclear.
In the present study, we used enzyme-linked immunosorbent assays (ELISA) to measure the production of cytokines following minocycline treatment of lipopolysaccharide- (LPS-) stimulated THP-1 cells.
Western blotting analysis was performed to confirm autophagy and the mTOR signal pathway.
Cell proliferation was measured by WST-1 cell proliferation assay.
We demonstrated that LPS induced autophagy in a tumor necrosis factor- (TNF-) α-mediated manner, and simultaneously, LPS induced the release of TNF-α to trigger inflammation and activated mammalian target of rapamycin (mTOR) to potentiate cell proliferation.
Minocycline, which induces autophagy by inhibiting mTOR, suppresses cytokine production and cell proliferation and protects THP-1 cells from LPS toxicity.
Further study demonstrated that there might be an intimate crosstalk between the inhibitor kappa B kinase (IKK)/nuclear factor-kappa B (NF-κB) signaling pathway and autophagy flux in modification of inflammatory responses.
In addition, rapamycin, the mTOR inhibitor, has cooperative effect with minocycline on suppression of TNF-α release and induction of autophagy by repressing mTOR.
Our data brought a novel clue to evaluate minocycline using as a potential therapeutic medicine for sepsis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Jian& Shigemi, Hiroko& Cao, Miaoyin& Qin, E.& Tang, Jixian& Shen, Juxin…[et al.]. 2020. Minocycline Induces Autophagy and Inhibits Cell Proliferation in LPS-Stimulated THP-1 Cells. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1134786
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Jian…[et al.]. Minocycline Induces Autophagy and Inhibits Cell Proliferation in LPS-Stimulated THP-1 Cells. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1134786
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Jian& Shigemi, Hiroko& Cao, Miaoyin& Qin, E.& Tang, Jixian& Shen, Juxin…[et al.]. Minocycline Induces Autophagy and Inhibits Cell Proliferation in LPS-Stimulated THP-1 Cells. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1134786
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134786
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر