Reducing Neuron Apoptosis in the Pontine Micturition Center by Nerve Root Transfer for Restoration of Micturition Function after Spinal Cord Injury
المؤلفون المشاركون
Yu, Ronghua
Yin, Gang
Zhao, Jianguo
Chen, Huihao
Meng, Depeng
Zhang, Jiaqiang
Lin, Yaofa
Xie, Zheng
Hou, Chunlin
Lin, Haodong
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-04
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objective.
The rate of neuronal apoptosis increases after spinal cord injury (SCI).
Anastomosing the normal nerve roots above the SCI level to the injured sacral nerve roots can enhance the functional recovery of neurons.
Therefore, we evaluated the effect of sacral nerve root transfer after SCI on pontine neuronal survival.
Methods.
Sprague–Dawley rats were randomly divided into three groups: Group A, reconstruction of afferent and efferent nerve pathways of the bladder after SCI; Group B, SCI only; and Group C, control group.
We examined pontine neuronal morphology using hematoxylin and eosin (H&E) staining after SCI and nerve transfer.
Bcl-2 and Bax protein expression changes in the pontine micturition center were quantified by immunohistochemistry.
The number of apoptotic neurons was determined by TUNEL staining.
We examined pontine neuronal apoptosis by transmission electron microscopy (TEM) at different time points.
Results.
H&E staining demonstrated that the number of neurons had increased in Group A, but more cells in Group B displayed nuclear pyknosis, with the disappearance of the nucleus.
Compared with Group B, Group A had significantly higher Bcl-2 expression, significantly lower Bax expression, and a significantly higher Bcl-2/Bax ratio.
The number of apoptotic neurons and neuron bodies in Group A was significantly lower than that in Group B, as indicated by TUNEL staining and TEM.
Conclusions.
These findings demonstrate that lumbosacral nerve transfer can reduce neuronal apoptosis in the pontine micturition center and enhance functional recovery of neurons.
This result further suggests that lumbosacral nerve transfer can be used as a new approach for reconstructing bladder function after spinal cord injury.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yu, Ronghua& Yin, Gang& Zhao, Jianguo& Chen, Huihao& Meng, Depeng& Zhang, Jiaqiang…[et al.]. 2020. Reducing Neuron Apoptosis in the Pontine Micturition Center by Nerve Root Transfer for Restoration of Micturition Function after Spinal Cord Injury. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134837
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yu, Ronghua…[et al.]. Reducing Neuron Apoptosis in the Pontine Micturition Center by Nerve Root Transfer for Restoration of Micturition Function after Spinal Cord Injury. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1134837
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yu, Ronghua& Yin, Gang& Zhao, Jianguo& Chen, Huihao& Meng, Depeng& Zhang, Jiaqiang…[et al.]. Reducing Neuron Apoptosis in the Pontine Micturition Center by Nerve Root Transfer for Restoration of Micturition Function after Spinal Cord Injury. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134837
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134837
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر