The Regulation of circRNA RNF13miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia
المؤلفون المشاركون
Zhang, Rong
Li, Yingchun
Wang, Hongtao
Zhu, Ke
Zhang, Guojun
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-06
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Objective.
To study the biological function of circular RNA RNF13 (circRNF13) in acute myeloid leukemia (AML) and its relationship with prognosis.
Methods.
We constructed stable AML cell lines with downregulated expression of circRNF13, and then, we explored the effect of downregulation of circRNF13 expression on the proliferation, migration, and invasion through qRT-PCR, MTT curve, colony formation, transwell migration and invasion experiment, cell cycle, apoptosis, Caspase 3/7 assay, and other experiments.
We also studied the expression of C-myc and Tenascin-C by qRT-PCR to explore the role of circRNF13.
Results.
When the expression of circRNF13 was downregulated, the proliferation rate of AML cells decreased significantly, the cell cycle was blocked to G1 phase, and apoptosis rate increased significantly.
C-myc related to cell proliferation decreased significantly at RNA level.
Furthermore, when the expression of circRNF13 was downregulated, the migration and invasion ability of AML cells was significantly reduced, and the expression of Tenascin-C related to migration and invasion also decreased significantly.
The luciferase reporter assay system confirmed that miRNA-1224-5p was the direct target of circRNF13.
Conclusion.
CircRNF13 inhibited the proliferation, migration, and invasion of AML cells by regulating the expression of miRNA-1224-5p.
This study provides some clues for the diagnosis and treatment of AML.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Rong& Li, Yingchun& Wang, Hongtao& Zhu, Ke& Zhang, Guojun. 2020. The Regulation of circRNA RNF13miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1134862
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Rong…[et al.]. The Regulation of circRNA RNF13miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1134862
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Rong& Li, Yingchun& Wang, Hongtao& Zhu, Ke& Zhang, Guojun. The Regulation of circRNA RNF13miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1134862
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134862
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر