Decreased ALKBH5, FTO, and YTHDF2 in Peripheral Blood Are as Risk Factors for Rheumatoid Arthritis
المؤلفون المشاركون
Luo, Qing
Gao, Yujie
Zhang, Lu
Rao, Jiayue
Guo, Yang
Huang, Zikun
Li, Junming
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-20
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
ALKBH5 (alkylation repair homolog protein 5), FTO (fat mass and obesity-associated protein), and RNA N6-methyladenosine (m6A) demethylase, are essential for the m6A mRNA modification.
YTHDF2 (YT521-B homology domains 2) called m6A “readers” can recognize m6A modification.
As the key enzymes of m6A methylation modification, ALKBH5, FTO, and YTHDF2 have been implicated in many diseases.
However, little is known about the role of ALKBH5, FTO, and YTHDF2 in rheumatoid arthritis (RA).
We measured the mRNA expression of ALKBH5, FTO, and YTHDF2 in RA patients and controls by quantitative real-time polymerase chain reaction, and the global m6A content was detected by an ELISA-like format.
The mRNA expression of ALKBH5, FTO, and YTHDF2 in RA patients was further analyzed to investigate its correlations with disease activity.
And, multivariate analysis (logistic regression) was used to analyze the risk factors.
The mRNA expression of ALKBH5, FTO, and YTHDF2 in RA patients was significantly decreased compared to controls.
The mRNA expression of ALKBH5 was significantly increased in RA patients that received regular treatment.
The mRNA expression of FTO was associated with disease activity score 28 (DAS28), complement 3 (C3), immunoglobulin G (IgG), and lymphocyte-to-monocyte ratio (LMR), some common markers for RA disease activity.
The mRNA expression of YTHDF2 was associated with RBC, L%, N%, NLR, and LMR.
Furthermore, logistic regression analysis revealed that decreased expression of ALKBH5, FTO, and YTHDF2 in peripheral blood was a risk factor for RA.
Moreover, the peripheral blood global m6A content was significantly increased in patients with RA compared to CON, and increased m6A contents negatively correlated with decreased mRNA expression of FTO.
In conclusion, this study firstly demonstrates the critical role of ALKBH5, FTO, and YTHDF2 in RA, which provides novel insights into recognizing the pathogenesis of RA and a promising biomarker for RA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Luo, Qing& Gao, Yujie& Zhang, Lu& Rao, Jiayue& Guo, Yang& Huang, Zikun…[et al.]. 2020. Decreased ALKBH5, FTO, and YTHDF2 in Peripheral Blood Are as Risk Factors for Rheumatoid Arthritis. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134903
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Luo, Qing…[et al.]. Decreased ALKBH5, FTO, and YTHDF2 in Peripheral Blood Are as Risk Factors for Rheumatoid Arthritis. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1134903
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Luo, Qing& Gao, Yujie& Zhang, Lu& Rao, Jiayue& Guo, Yang& Huang, Zikun…[et al.]. Decreased ALKBH5, FTO, and YTHDF2 in Peripheral Blood Are as Risk Factors for Rheumatoid Arthritis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134903
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134903
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر