Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data
المؤلفون المشاركون
Yi, Shanyong
Lin, Qianwen
Zhang, Xuejia
Wang, Jing
Miao, Yuanyuan
Tan, Ninghua
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-09
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Real-time quantitative polymerase chain reaction (RT-qPCR) has been widely applied in gene expression and transcription abundance analysis because of its high sensitivity, good repeatability, and strong specificity.
Selection of relatively stable reference genes is a precondition in order to obtain the reliable analysis results.
However, little is known about evaluation of a set of reference genes through scientific experiments in Rubia plants.
Here, 15 candidate reference genes were selected from R.
yunnanensis transcriptome database and analyzed under abiotic stresses, hormone treatments, and different tissues.
Among these 15 candidate reference genes, heterogeneous nuclear ribonucleoprotein (hnRNP), TATA binding protein (TBP), ribosomal protein L5 (RPL5), malate dehydrogenase (MDH), and elongation factor 1-alpha (EF-1α) were indicated as the five most stable reference genes by four statistical programs (geNorm, NormFinder, BestKeeper, and RefFinder).
Ultimately, the validity of reference genes was confirmed by normalizing the expression of o-succinylbenzoate-CoA ligase (OSBL) and isochorismate synthase (ICS) involved in the anthraquinone biosynthesis pathway in different tissues and hormone treatments.
Meanwhile, four other putative genes involved in the anthraquinone biosynthesis pathway were also normalized with the selected reference genes, which showed similar expression levels with those given by transcriptome data.
This work is the first research that aims at a systematic validation on the stability of reference genes selected from R.
yunnanensis transcriptome data and will be conducive to analyze gene expression in R.
yunnanensis or other Rubia species.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yi, Shanyong& Lin, Qianwen& Zhang, Xuejia& Wang, Jing& Miao, Yuanyuan& Tan, Ninghua. 2020. Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data. BioMed Research International،Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1134968
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yi, Shanyong…[et al.]. Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data. BioMed Research International No. 2020 (2020), pp.1-19.
https://search.emarefa.net/detail/BIM-1134968
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yi, Shanyong& Lin, Qianwen& Zhang, Xuejia& Wang, Jing& Miao, Yuanyuan& Tan, Ninghua. Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1134968
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134968
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر