Let-7c-3p Regulates Autophagy under Oxidative Stress by Targeting ATG3 in Lens Epithelial Cells
المؤلفون المشاركون
Huang, Yan-Hong
Li, Ting
Zhou, Wenkai
Yan, Qichang
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-16
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
Oxidative stress is an important factor during age-related cataract formation.
Apoptosis and autophagy induced by oxidative stress have been reported as key factors in age-related cataract.
In our research, we investigated the role of let-7c-3p in the regulation of autophagy and apoptosis during the formation of age-related cataract.
Material and Methods.
Real-time PCR and western blot were employed to detect the expression of let-7c-3p in the tissues of age-related cataract.
Human lens epithelial cells (LECs) were treated with H2O2 as an age-related cataract model.
The extent of apoptosis was measured by flow cytometry and western blot.
To detect autophagy, immunofluorescence was used to analyze the spot number of LC3, and western blot was used to detect the expression of LC3-II/I and ATG3.
The molecular mechanisms of let-7c-3p regulating autophagy via ATG3 under oxidative stress were performed by a luciferase report gene assay and rescue experiment.
Results.
Downregulation of let-7c-3p was found in the age-related cataract group aged >65 years relative to the age-related cataract group aged ≤65 years.
Consistently, the expression of let-7c-3p was also lower under oxidative stress.
The activities of LEC apoptosis and autophagy induced by oxidative stress were inhibited by let-7c-3p.
By the bioinformatics database and the luciferase reporter assay, ATG3 was found to be a direct target of let-7c-3p.
Let-7c-3p reduced the ATG3-mediated autophagy level, which was induced by oxidative stress in LECs.
Conclusion.
Let-7c-3p inhibits autophagy by targeting ATG3 in LECs in age-related cataract.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Ting& Huang, Yan-Hong& Zhou, Wenkai& Yan, Qichang. 2020. Let-7c-3p Regulates Autophagy under Oxidative Stress by Targeting ATG3 in Lens Epithelial Cells. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1135467
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Ting…[et al.]. Let-7c-3p Regulates Autophagy under Oxidative Stress by Targeting ATG3 in Lens Epithelial Cells. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1135467
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Ting& Huang, Yan-Hong& Zhou, Wenkai& Yan, Qichang. Let-7c-3p Regulates Autophagy under Oxidative Stress by Targeting ATG3 in Lens Epithelial Cells. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1135467
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135467
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر