Isolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp
المؤلفون المشاركون
Weng, Cheng-Hao
Yen, Tzung-Hai
Huang, Wen-Hung
Wang, I-Kwan
Tsai, Aileen I.
Lin, Wey-Ran
Chang, Chih-Chun
Hsu, Ching-Wei
Hong, Hsiang-Hsi
Fu, Jen-Fen
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-09
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
This study aimed to identify predictors of success rate of mesenchymal stem cell (MSC) isolation from human deciduous teeth pulp.
A total of 161 deciduous teeth were extracted at the dental clinic of Chang Gung Memorial Hospital.
The MSCs were isolated from dental pulps using a standard protocol.
In total, 128 colonies of MSCs were obtained and the success rate was 79.5%.
Compared to teeth not yielding MSCs successfully, those successfully yielding MSCs were found to have less severe dental caries (no/mild-to-moderate/severe: 63.3/24.2/12.5% versus 12.5/42.4/42.4%, P<0.001) and less frequent pulpitis (no/yes: 95.3/4.7% versus 51.5/48.5%, P<0.001).
In a multivariate regression model, it was confirmed that the absence of dental caries (OR = 4.741, 95% CI = 1.564–14.371, P=0.006) and pulpitis (OR = 9.111, 95% CI = 2.921–28.420, P<0.001) was significant determinants of the successful procurement of MSCs.
MSCs derived from pulps with pulpitis expressed longer colony doubling time than pulps without pulpitis.
Furthermore, there were higher expressions of proinflammatory cytokines, interleukin- (IL-) 6 and monocyte chemoattractant protein- (MCP-) 1, P<0.01, and innate immune response [toll-like receptor 1 (TLR1) and TLR8, P<0.05; TLR2, TLR3, and TLR6, P<0.01] in the inflamed than noninflamed pulps.
Therefore, a carious deciduous tooth or tooth with pulpitis was relatively unsuitable for MSC processing and isolation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tsai, Aileen I.& Hong, Hsiang-Hsi& Lin, Wey-Ran& Fu, Jen-Fen& Chang, Chih-Chun& Wang, I-Kwan…[et al.]. 2017. Isolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1135477
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tsai, Aileen I.…[et al.]. Isolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1135477
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tsai, Aileen I.& Hong, Hsiang-Hsi& Lin, Wey-Ran& Fu, Jen-Fen& Chang, Chih-Chun& Wang, I-Kwan…[et al.]. Isolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1135477
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135477
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر