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Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro
المؤلفون المشاركون
Dolo, Vincenza
Lombardi, Francesca
Palumbo, Paola
Augello, Francesca Rosaria
Guerrini, Luca
Cifone, Maria Grazia
Giuliani, Maurizio
Cinque, Benedetta
Giusti, Ilaria
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-26
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The ability of a collagen-based matrix to support cell proliferation, migration, and infiltration has been reported; however, the direct effect of an aqueous collagen suspension on cell cultures has not been studied yet.
In this work, the effects of a high-concentration aqueous suspension of a micronized type I equine collagen (EC-I) have been evaluated on a normal mouse fibroblast cell line.
Immunofluorescence analysis showed the ability of EC-I to induce a significant increase of type I and III collagen levels, parallel with overexpression of crucial proteins in collagen biosynthesis, maturation, and secretion, prolyl 4-hydroxylase (P4H) and heat shock protein 47 (HSP47), as demonstrated by western blot experiments.
The treatment led, also, to an increase of α-smooth muscle actin (α-SMA) expression, evaluated through western blot analysis, and cytoskeletal reorganization, as assessed by phalloidin staining.
Moreover, scanning electron microscopy analysis highlighted the appearance of plasma membrane extensions and blebbing of extracellular vesicles.
Altogether, these results strongly suggest that an aqueous collagen type I suspension is able to induce fibroblast myodifferentiation.
Moreover, our findings also support in vitro models as a useful tool to evaluate the effects of a collagen suspension and understand the molecular signaling pathways possibly involved in the effects observed following collagen treatment in vivo.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lombardi, Francesca& Palumbo, Paola& Augello, Francesca Rosaria& Giusti, Ilaria& Dolo, Vincenza& Guerrini, Luca…[et al.]. 2020. Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1135484
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lombardi, Francesca…[et al.]. Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1135484
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lombardi, Francesca& Palumbo, Paola& Augello, Francesca Rosaria& Giusti, Ilaria& Dolo, Vincenza& Guerrini, Luca…[et al.]. Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1135484
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135484
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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