Impact of Prolonged Mechanical Ventilation on Ferroptosis in Renal IschemiaReperfusion Injury in Rats
المؤلفون المشاركون
Chen, Jianghua
Yang, Yi
Zhou, Fangfang
Luo, Lianxin
Chen, Yixin
Luo, Qun
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-24
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
We here investigated the impact of mechanical ventilation (MV) time on ferroptosis in a rat renal ischemia/reperfusion injury (IRI) model.
Thirty-two male adult Sprague Dawley rats were divided into four groups (n=8/group): the sham group, IRI group, IRI+MV-4 h group, and IRI+MV-12 h group.
Rats in the IRI group were subjected to 45 min bilateral renal ischemia.
Rats in the IRI+MV groups were additionally mechanically ventilated with tracheal intubation after 45 min bilateral renal ischemia.
Morphological changes associated with kidney injury and ferroptosis were assessed by hematoxylin and eosin staining and electron microscopy.
Levels of the central regulator of ferroptosis, glutathione peroxidase 4 (GPX4), and lipid peroxidation markers 4-hydroxynonenal (4HNE) and superoxide dismutase 2 (SOD2) were determined in the kidney tissue by western blotting.
Glutathione (GSH) levels were assessed in the serum and kidney homogenate.
Scr levels in the IRI+MV-12 h group were significantly higher than those in the sham, IRI, and IRI+MV-4 h groups (all P<0.001).
Electron microscopy revealed the most pronouncedly abnormal mitochondrial morphology, suggestive of ferroptosis, in the IRI+MV-12 h group.
The GPX4 and SOD2 protein levels progressively decreased in the following order: sham group > IRI group > IRI+MV-4 h group > IRI+MV-12 h group (P<0.05 for all comparisons).
By contrast, the 4HNE levels progressively increased in the kidney, with the highest values in the IRI+MV-12 h group (P<0.05, vs.
the IRI group and vs.
the IRI+MV-4 h group).
Further, the GSH levels in the serum and kidney homogenates were significantly reduced in the IRI+MV-12 h group (P<0.01, vs.
IRI group and vs.
the IRI+MV-4 h group).
A significant positive correlation was observed between the serum and kidney GSH levels (r2=0.542, P=0.03).
These observations suggested that prolonged MV may exacerbate renal function failure, already initiated by IRI, by ferroptosis.
Depletion of GSH may contribute to this effect, which requires further investigation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Fangfang& Yang, Yi& Luo, Lianxin& Chen, Yixin& Luo, Qun& Chen, Jianghua. 2020. Impact of Prolonged Mechanical Ventilation on Ferroptosis in Renal IschemiaReperfusion Injury in Rats. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1135497
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Fangfang…[et al.]. Impact of Prolonged Mechanical Ventilation on Ferroptosis in Renal IschemiaReperfusion Injury in Rats. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1135497
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Fangfang& Yang, Yi& Luo, Lianxin& Chen, Yixin& Luo, Qun& Chen, Jianghua. Impact of Prolonged Mechanical Ventilation on Ferroptosis in Renal IschemiaReperfusion Injury in Rats. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1135497
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135497
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر