Deletion of Akt1 Promotes Kidney Fibrosis in a Murine Model of Unilateral Ureteral Obstruction
المؤلفون المشاركون
Lee, Min Young
Kim, Il Young
Park, Mi Wha
Seong, Eun Young
Lee, Dong Won
Lee, Soo Bong
Bae, Sun Sik
Kim, Sang Soo
Song, Sang Heon
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-24
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
We investigated the role of Akt1, one of the three isoforms of Akt, in renal fibrosis using the murine model of unilateral ureteral obstruction (UUO).
We subjected wild type and Akt1−/− mice to UUO.
The Akt1 gene was silenced in vitro using short hairpin RNA delivered via a lentiviral vector in human proximal tubular cells (HK2 cells) and kidney fibroblasts (NRK-49F cells).
The obstructive kidneys of Akt1−/− mice showed more severe tubulointerstitial fibrosis than those of wild type mice.
The expression of fibronectin and type I collagen was significantly increased in obstructed kidneys of Akt1−/− mice compared to those of wild type mice.
The important finding was that the expression of transforming growth factor β1 (TGFβ1) was significantly increased in the Akt1−/− mice compared to the wild type mice.
The knockdown of Akt1 enhanced the expression of TGFβ1 in HK2 cells.
Interestingly, the upregulation of TGFβ1 due to genetic knockdown of Akt1 was associated with activation of signal transducer and activator of transcript 3 (STAT3) independently of the Smad pathway in NRK-49F and HK2 cells.
Immunohistochemical staining also showed that expression of phosphorylated STAT3 was more increased in Akt1−/− mice than in wild type mice after UUO.
Additionally, the deletion of Akt1 led to apoptosis of the renal tubular cells in both in vivo and in vitro studies.
Conclusively, these results suggest that the deletion of Akt1 may contribute to renal fibrosis via induction of the TGFβ1/STAT3 pathway in a murine model of UUO.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Il Young& Lee, Min Young& Park, Mi Wha& Seong, Eun Young& Lee, Dong Won& Lee, Soo Bong…[et al.]. 2020. Deletion of Akt1 Promotes Kidney Fibrosis in a Murine Model of Unilateral Ureteral Obstruction. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1135548
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Il Young…[et al.]. Deletion of Akt1 Promotes Kidney Fibrosis in a Murine Model of Unilateral Ureteral Obstruction. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1135548
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Il Young& Lee, Min Young& Park, Mi Wha& Seong, Eun Young& Lee, Dong Won& Lee, Soo Bong…[et al.]. Deletion of Akt1 Promotes Kidney Fibrosis in a Murine Model of Unilateral Ureteral Obstruction. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1135548
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135548
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر