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3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity
المؤلفون المشاركون
Peng, Qing
Gao, Yi
Li, Yang
Jiang, Xinan
Jia, Zhidong
Cheng, Yuan
Zhang, Chengyan
Wang, Gaoshang
Xu, Jiecheng
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-22، 22ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-04
دولة النشر
مصر
عدد الصفحات
22
التخصصات الرئيسية
الملخص EN
In vitro 3D hepatocyte culture constitutes a core aspect of liver tissue engineering.
However, conventional 3D cultures are unable to maintain hepatocyte polarity, functional phenotype, or viability.
Here, we employed microfluidic chip technology combined with natural alginate hydrogels to construct 3D liver tissues mimicking hepatic plates.
We comprehensively evaluated cultured hepatocyte viability, function, and polarity.
Transcriptome sequencing was used to analyze changes in hepatocyte polarity pathways.
The data indicate that, as culture duration increases, the viability, function, polarity, mRNA expression, and ultrastructure of the hepatic plate mimetic 3D hepatocytes are enhanced.
Furthermore, hepatic plate mimetic 3D cultures can promote changes in the bile secretion pathway via effector mechanisms associated with nuclear receptors, bile uptake, and efflux transporters.
This study provides a scientific basis and strong evidence for the physiological structures of bionic livers prepared using 3D cultures.
The systems and cultured liver tissues described here may serve as a better in vitro 3D culture platform and basic unit for varied applications, including drug development, hepatocyte polarity research, bioartificial liver bioreactor design, and tissue and organ construction for liver tissue engineering or cholestatic liver injury.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jia, Zhidong& Cheng, Yuan& Jiang, Xinan& Zhang, Chengyan& Wang, Gaoshang& Xu, Jiecheng…[et al.]. 2020. 3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity. BioMed Research International،Vol. 2020, no. 2020, pp.1-22.
https://search.emarefa.net/detail/BIM-1135769
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jia, Zhidong…[et al.]. 3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity. BioMed Research International No. 2020 (2020), pp.1-22.
https://search.emarefa.net/detail/BIM-1135769
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jia, Zhidong& Cheng, Yuan& Jiang, Xinan& Zhang, Chengyan& Wang, Gaoshang& Xu, Jiecheng…[et al.]. 3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-22.
https://search.emarefa.net/detail/BIM-1135769
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135769
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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