Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-Resistant Klebsiella pneumoniae
المؤلفون المشاركون
Siddique, Muhammad Hussnain
Aslam, Bilal
Imran, Muhammad
Ashraf, Asma
Nadeem, Habibullah
Hayat, Sumreen
Khurshid, Mohsin
Afzal, Muhammad
Malik, Imran Riaz
Shahzad, Mudassar
Qureshi, Umber
Khan, Zia Ul Haq
Muzammil, Saima
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-20
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Antibiotic resistance against present antibiotics is rising at an alarming rate with need for discovery of advanced methods to treat infections caused by resistant pathogens.
Silver nanoparticles are known to exhibit satisfactory antibacterial and antibiofilm activity against different pathogens.
In the present study, the AgNPs were synthesized chemically and characterized by UV-Visible spectroscopy, scanning electron microscopy, and X-ray diffraction.
Antibacterial activity against MDR K.
pneumoniae strains was evaluated by agar diffusion and broth microdilution assay.
Cellular protein leakage was determined by the Bradford assay.
The effect of AgNPs on production on extracellular polymeric substances was evaluated.
Biofilm formation was assessed by tube method qualitatively and quantitatively by the microtiter plate assay.
The cytotoxic potential of AgNPs on HeLa cell lines was also determined.
AgNPs exhibited an MIC of 62.5 and 125 μg/ml, while their MBC is 250 and 500 μg/ml.
The production of extracellular polymeric substance decreased after AgNP treatment while cellular protein leakage increased due to higher rates of cellular membrane disruption by AgNPs.
The percentage biofilm inhibition was evaluated to be 64% for K.
pneumoniae strain MF953600 and 86% for MF953599 at AgNP concentration of 100 μg/ml.
AgNPs were evaluated to be minimally cytotoxic and safe at concentrations of 15-120 μg/ml.
The data evaluated by this study provided evidence of AgNPs being safe antibacterial and antibiofilm compounds against MDR K.
pneumoniae.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Siddique, Muhammad Hussnain& Aslam, Bilal& Imran, Muhammad& Ashraf, Asma& Nadeem, Habibullah& Hayat, Sumreen…[et al.]. 2020. Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-Resistant Klebsiella pneumoniae. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1135819
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Siddique, Muhammad Hussnain…[et al.]. Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-Resistant Klebsiella pneumoniae. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1135819
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Siddique, Muhammad Hussnain& Aslam, Bilal& Imran, Muhammad& Ashraf, Asma& Nadeem, Habibullah& Hayat, Sumreen…[et al.]. Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-Resistant Klebsiella pneumoniae. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1135819
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135819
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر