Isolation of Potential Phages against Multidrug-Resistant Bacterial Isolates: Promising Agents in the Rivers of Kathmandu, Nepal
المؤلفون المشاركون
Parajuli, Narayan Prasad
Khanal, Puspa Raj
Satyal, Deepa
Bhetwal, Anjeela
Maharjan, Anjila
Shakya, Shreena
Ghimire, Sumitra
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-22
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Bacteriophages are being the subject of interest for alternative antimicrobial therapy for infectious diseases in recent years.
Therapeutic effectiveness regarding phage therapy is a matter of concern since it is the most promising biological treatment of this era.
Hence, the present study was aimed to isolate the potential bacteriophages present in river water samples and to analyze their host range among clinical strains of bacteria.
Ten different locations of Kathmandu valley were selected for the collection of river water for the detection of probable phages.
Bacteriophages were isolated from water samples using the double agar overlay method.
Isolated phages were purified by diluting in the SM-buffer and filtering through 0.22 μm filter.
Purified lysate was further processed for analyzing its host range by using spot method.
Their host range was characterized against 20 bacterial strains, including multidrug-resistant.
Total 67 different phages were isolated against 8 different host organisms.
Out of them, forty-seven phages were selected for analyzing its host range.
Among them, Serratia phages (ΦSER) had the broad host range infecting 17 different bacterial strains including multidrug-resistant harboring ESBL and MBL genotypes.
However, Klebsiella phages (ΦKP) had narrow host range in comparison to other phages.
Isolated phages had the potential effect against clinical strains of bacteria along with their broader host spectrum.
Most importantly, promising effect against MDR pathogens in this study has raised the probable chances of the utility of these phages for biological control of bacterial infection including MBL and ESBL strains.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bhetwal, Anjeela& Maharjan, Anjila& Shakya, Shreena& Satyal, Deepa& Ghimire, Sumitra& Khanal, Puspa Raj…[et al.]. 2017. Isolation of Potential Phages against Multidrug-Resistant Bacterial Isolates: Promising Agents in the Rivers of Kathmandu, Nepal. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1136301
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bhetwal, Anjeela…[et al.]. Isolation of Potential Phages against Multidrug-Resistant Bacterial Isolates: Promising Agents in the Rivers of Kathmandu, Nepal. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1136301
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bhetwal, Anjeela& Maharjan, Anjila& Shakya, Shreena& Satyal, Deepa& Ghimire, Sumitra& Khanal, Puspa Raj…[et al.]. Isolation of Potential Phages against Multidrug-Resistant Bacterial Isolates: Promising Agents in the Rivers of Kathmandu, Nepal. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1136301
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136301
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر