RANKL Is Involved in Runx2-Triggered Hepatic Infiltration of Macrophages in Mice with NAFLD Induced by a High-Fat Diet
المؤلفون المشاركون
Zhong, Li
Yuan, Jianghan
Huang, Lu
Li, Shan
Deng, Liang
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-27
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background.
Receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL) is significant in the activation of inflammation.
Runt-related transcription factor 2 (Runx2) promotes the hepatic infiltration of macrophages in nonalcoholic fatty liver disease (NAFLD).
We studied how RANKL affects Runx2-triggered macrophage infiltration in NAFLD.
Method.
30 male C57BL/6J mice at 4 weeks of age were utilized in this study, 20 mice received a high-fat diet (HFD), and 10 mice received standard rodent chow over 8 months.
The histopathologic features of the liver were identified by H&E, Oil red O, and Masson staining.
Runx2, RANKL, and F4/80 were analyzed by western blot, real-time PCR, and immunohistochemistry in vivo, respectively.
Lentivirus or siRNA was utilized for transwell assay to investigate the role of RANKL in Runx2-induced macrophage migration in vitro.
Results.
Compared to controls, during NAFLD development, HFD increased Runx2 and RANKL in vivo in NASH (P<0.01).
Meanwhile, a correlation between the expression of Runx2 and RANKL (P<0.05) was evident.
In addition, the hepatic infiltration of macrophages was increased upon HFD feeding, and analysis showed that the macrophage infiltration was correlated with the expression of Runx2 or RANKL (P<0.05).
In vitro, we found that overexpression or deficiency of Runx2 increased or decreased the production of RANKL in mHSCs.
Then, transwell assay revealed that RANKL was involved in Runx2-induced macrophage migration.
Conclusions.
Overall, RANKL is involved in Runx2-triggered macrophage migration during NAFLD pathogenesis, which may provide an underlying therapeutic target for NAFLD.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhong, Li& Yuan, Jianghan& Huang, Lu& Li, Shan& Deng, Liang. 2020. RANKL Is Involved in Runx2-Triggered Hepatic Infiltration of Macrophages in Mice with NAFLD Induced by a High-Fat Diet. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1136361
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhong, Li…[et al.]. RANKL Is Involved in Runx2-Triggered Hepatic Infiltration of Macrophages in Mice with NAFLD Induced by a High-Fat Diet. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1136361
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhong, Li& Yuan, Jianghan& Huang, Lu& Li, Shan& Deng, Liang. RANKL Is Involved in Runx2-Triggered Hepatic Infiltration of Macrophages in Mice with NAFLD Induced by a High-Fat Diet. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1136361
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136361
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر