Let-7c-5p Is Involved in Chronic Kidney Disease by Targeting TGF-β Signaling
المؤلفون المشاركون
Wang, Zhenguo
Zhou, Cuixing
Sun, Yangyang
Chen, Yimeng
Xue, Dong
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-13
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The purpose of the present study was to investigate the expressions of hsa-let-7c-5p and TGF-β signaling-related molecules and their correlations with clinical characteristics in chronic kidney disease (CKD).
Twenty-three biopsy specimens of CKD patients and 20 negative control tissues were selected.
Quantitative real-time PCR (qPCR) was used for the detection of hsa-let-7c-5p, transforming growth factor β (TGF-β) and TGF-β receptor type 1 (TGF-βR1) expression levels.
Target gene of hsa-let-7c-5p was verified by dual-luciferase reporter assay.
A significant decrease of hsa-let-7c-5p expression in CKD tissue was found, compared with that of normal renal tissues (p<0.01).
Expression levels of TGF-β in CKD were increased, compared with that of normal kidney tissue (p<0.001).
The difference in the expression of TGF-β R1 between CKD tissues and normal renal tissues was not significant (p>0.05).
A negative correlation was found between the expression of TGF-β and renal tissue hsa-let-7c-5p levels.
Furthermore, hsa-let-7c-5p was identified to regulate TGF- β1 by directly binding with the 167-173 site in the 3′ untranslated region.
Decreased hsa-let-7c-5p levels in CKD patients was found to be associated with disease severity, which shows a negative correlation with proteinuria and creatinine levels, and a positive correlation with estimated glomerular filtration rate (eGFR), while relative TGF-β1 expression had a positive correlation with creatinine level.
In summary, changes in hsa-let-7c-5p expression and its target gene TGF-β are associated with the disease status of CKD.
Let-7c-5p may contribute to the pathogenesis of renal fibrosis through TGF-β signaling, a potential diagnostic and therapeutic target of the disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Zhenguo& Zhou, Cuixing& Sun, Yangyang& Chen, Yimeng& Xue, Dong. 2020. Let-7c-5p Is Involved in Chronic Kidney Disease by Targeting TGF-β Signaling. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1136372
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Zhenguo…[et al.]. Let-7c-5p Is Involved in Chronic Kidney Disease by Targeting TGF-β Signaling. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1136372
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Zhenguo& Zhou, Cuixing& Sun, Yangyang& Chen, Yimeng& Xue, Dong. Let-7c-5p Is Involved in Chronic Kidney Disease by Targeting TGF-β Signaling. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1136372
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136372
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر