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Expression and Role of Oct34 in Injury-Repair Process of Rat Alveolar Epithelium after 5-Fu Treatment
المؤلفون المشاركون
Jia, Xinshan
Li, Wen-ya
Ye, Xu-lv
Jia, Lan-ling
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-08-29
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Objective.
We aimed to investigate how the embryonic stem cell-related gene Oct3/4 changes during the injury-repair process of distal pulmonary epithelium induced by 5-fluorouracil (5-Fu).
Methods.
We have developed the lung injury model induced by 5-Fu and observed the dynamic changes of Oct3/4 by indirect immunofluorescence, Western blot, and quantitative real-time PCR.
Immunofluorescence double staining was used to compare the positions of Oct3/4(+) cells and other reported alveolar epithelial stem cells.
Results.
Oct3/4(+) cells were not found in normal rat lung epithelial cells.
However, after treatment with 5-Fu, Oct3/4(+) cells appeared at 12 h, reached the peak at 24 h, then decreased at 48 h, and eventually disappeared at 72 h.
Oct3/4 was localized in the nucleus.
We found that the sites of Clara cell secretory protein and surfactant protein-C dual positive cells were apparently different from Oct3/4(+) cells.
Conclusions.
Our results revealed that, in rat alveolar epithelium, expression of Oct3/4 could be induced after treatment with 5-Fu, then decreased gradually, and was silenced following the alveolar epithelial differentiation.
We hold that Oct3/4(+) cells are lung stem cells, which can provide new evidence for identification and isolation of lung epithelial stem cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Wen-ya& Ye, Xu-lv& Jia, Xinshan& Jia, Lan-ling. 2017. Expression and Role of Oct34 in Injury-Repair Process of Rat Alveolar Epithelium after 5-Fu Treatment. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1136470
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Wen-ya…[et al.]. Expression and Role of Oct34 in Injury-Repair Process of Rat Alveolar Epithelium after 5-Fu Treatment. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1136470
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Wen-ya& Ye, Xu-lv& Jia, Xinshan& Jia, Lan-ling. Expression and Role of Oct34 in Injury-Repair Process of Rat Alveolar Epithelium after 5-Fu Treatment. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1136470
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136470
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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