Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model
المؤلفون المشاركون
Nowicki, Michał
Kempisty, Bartosz
Piotrowska, Hanna
Wojtanowicz-Markiewicz, Katarzyna
Kulus, Magdalena
Knap, Sandra
Kocherova, Ievgenia
Jankowski, Maurycy
Stefańska, Katarzyna
Jeseta, Michal
Bukowska, Dorota
Zabel, Maciej
Mozdziak, Paul
Antosik, Paweł
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-03
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes.
Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules.
In this work, we have examined how AQPs and Cx’s behave in luminal epithelium primary cell culture.
Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days.
Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR.
The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture.
Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture.
We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10.
The obtained results could become a point of reference for further in vivo and clinical research.
Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wojtanowicz-Markiewicz, Katarzyna& Kulus, Magdalena& Knap, Sandra& Kocherova, Ievgenia& Jankowski, Maurycy& Stefańska, Katarzyna…[et al.]. 2020. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. BioMed Research International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1136512
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wojtanowicz-Markiewicz, Katarzyna…[et al.]. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. BioMed Research International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1136512
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wojtanowicz-Markiewicz, Katarzyna& Kulus, Magdalena& Knap, Sandra& Kocherova, Ievgenia& Jankowski, Maurycy& Stefańska, Katarzyna…[et al.]. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1136512
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136512
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر