Identification of Streptococcus pneumoniae: Development of a Standardized Protocol for Optochin Susceptibility Testing Using Total Lab Automation
المؤلفون المشاركون
Burckhardt, Irene
Panitz, Jessica
Burckhardt, Florian
Zimmermann, Stefan
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-19
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Purpose.
Optochin susceptibility is one parameter used in the laboratory to identify Streptococcus pneumoniae.
However, a single standardized procedure does not exist.
Optochin is included neither in the current EUCAST breakpoint tables nor in the CLSI performance standards for antimicrobial susceptibility testing.
We wanted to establish an evidence-based protocol for optochin testing for our Total Lab Automation.
Methods.
We tested seven different agars and four different reading time points (7 h, 12 h, 18 h, and 24 h).
To accommodate for serotype diversity, all tests were done with 99 different strains covering 34 different serotypes of S.
pneumoniae.
We calculated a multivariable linear regression using data from 5544 inhibition zones.
Results.
Reading was possible for all strains at 12 h.
Agar type and manufacturer influenced the size of the inhibition zones by up to 2 mm and they varied considerably depending on serotype (up to 3 mm for serotype 3).
Depending on agar and reading time point, up to 38% of inhibition zones were smaller than the cut-off of 14 mm; that is, the result of the test was false-negative.
Conclusions.
Shortening incubation time from 24 h to 12 h for optochin susceptibility testing is feasible.
Agar and incubation time have to be chosen carefully to avoid false-negative results.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Burckhardt, Irene& Panitz, Jessica& Burckhardt, Florian& Zimmermann, Stefan. 2017. Identification of Streptococcus pneumoniae: Development of a Standardized Protocol for Optochin Susceptibility Testing Using Total Lab Automation. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1136822
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Burckhardt, Irene…[et al.]. Identification of Streptococcus pneumoniae: Development of a Standardized Protocol for Optochin Susceptibility Testing Using Total Lab Automation. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1136822
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Burckhardt, Irene& Panitz, Jessica& Burckhardt, Florian& Zimmermann, Stefan. Identification of Streptococcus pneumoniae: Development of a Standardized Protocol for Optochin Susceptibility Testing Using Total Lab Automation. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1136822
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136822
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر