Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor AlphaB-Cell Lymphoma 6 Pathway

المؤلفون المشاركون

Li, Ning
Meng, Dandan
Xu, Yue
Gao, Ling
Shen, Fengqian
Tie, Xiaojing
Zhang, Yan
Yi, Zhenying
Shen, Wenjie
Liu, Zonglan
Xu, Zhiqiao

المصدر

BioMed Research International

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-12-09

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

Gastric carcinoma is a common malignant cancer.

Pyruvate kinase M2 (PKM2) is highly expressed in cancers, including gastric carcinoma.

However, its function and molecular mechanism in gastric carcinoma remains unclear.

Here, we aimed to explore the function and the underlying mechanism of PKM2 on malignant phenotypes in gastric carcinoma.

In this study, the mRNA levels and protein levels of PKM2 in gastric carcinoma cell lines and normal gastric mucosa epithelial cell lines were detected using quantitative real-time PCR and western blot, respectively.

PKM2 was downregulated by siRNA transfection.

HIF-1α or BCL-6 was upregulated by corresponding overexpression plasmid.

Cell viability was detected using CCK-8 assay.

Cell invasion and migration were determined using transwell assay.

Higher expression of PKM2 was observed in human gastric carcinoma cell lines MKN-45 and SGC-7901 than in the normal gastric mucosa epithelial cell line GES-1.

PKM2 knockdown suppressed cancer cell invasion and migration and inhibited the epithelial-mesenchymal transition (EMT) phenotype by inhibiting E-cadherin and promoting vimentin and N-cadherin expression.

Also, we observed that PKM2 knockdown suppressed the hypoxia-inducible factor alpha (HIF-1α) and B-cell lymphoma 6 (BCL-6) signaling pathway.

HIF-1α overexpression reversed the function of PKM2 silencing on cell invasion, migration, EMT, and BCL-6 expression.

BCL-6 overexpression also reversed the function of PKM2 silencing on cell invasion, migration, and EMT but did not affect HIF-1α expression.

Taken together, data from our study suggest that PKM2 knockdown impeded cell migration, invasion, and EMT of gastric carcinoma cells via the HIF-1α/BCL-6 pathway.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Li, Ning& Meng, Dandan& Xu, Yue& Gao, Ling& Shen, Fengqian& Tie, Xiaojing…[et al.]. 2020. Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor AlphaB-Cell Lymphoma 6 Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1136897

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Li, Ning…[et al.]. Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor AlphaB-Cell Lymphoma 6 Pathway. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1136897

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Li, Ning& Meng, Dandan& Xu, Yue& Gao, Ling& Shen, Fengqian& Tie, Xiaojing…[et al.]. Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor AlphaB-Cell Lymphoma 6 Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1136897

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1136897