Nuclear Heme Oxidase-1 Inhibits Endoplasmic Reticulum Stress-Mediated Apoptosis after Spinal Cord Injury
المؤلفون المشاركون
Bi, Yunlong
Chen, Xi
Cao, Yang
Yu, Deshui
Zhao, Jia’ai
Jing, Yu
Lv, Gang
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-03
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The treatment goal for spinal cord injury (SCI) is to repair neurites and suppress cellular apoptosis.
This study is to investigate the effects of nuclear heme oxidase-1 (HO-1) on the acute spinal cord injury and the related mechanisms.
The rat model of the SCI was established.
On day 7, before model establishment, the adenovirus vector carrying nuclear HO-1 (Ad-GFP-HO-1CΔ23) was injected into the animals into the tenth thoracic spine (T10) segment by the intrathecal injection.
Starting from after the model establishment to day 28, the recovery of motor function was assessed by the Basso-Beattie-Bresnahan (BBB) scoring method.
Immunofluorescence was performed to detect the expression patterns of nuclear and cytoplasmic proteins.
HE and Nissl staining methods were used to evaluate the structural damage and the number of surviving neurons near the injured area.
The TUNEL method was conducted to evaluate the apoptotic degree.
Protein expression levels were detected with the Western blot analysis.
The BBB assay scores in the nuclear HO-1 group were significantly higher than the blank and adenovirus control groups.
Moreover, compared to the blank and adenovirus control groups, the neuronal apoptosis in the nuclear HO-1 group was significantly alleviated.
Furthermore, the expression levels of the endoplasmic reticulum stress-related proteins, i.e., CHOP, GRP78, and caspase-12, were significantly decreased in the nuclear HO-1 group.
Nuclear HO-1 significantly improves the SCI, promotes the functional recovery, inhibits the endoplasmic reticulum stress, and alleviates the apoptotic process after SCI.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bi, Yunlong& Chen, Xi& Cao, Yang& Yu, Deshui& Zhao, Jia’ai& Jing, Yu…[et al.]. 2020. Nuclear Heme Oxidase-1 Inhibits Endoplasmic Reticulum Stress-Mediated Apoptosis after Spinal Cord Injury. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1136987
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bi, Yunlong…[et al.]. Nuclear Heme Oxidase-1 Inhibits Endoplasmic Reticulum Stress-Mediated Apoptosis after Spinal Cord Injury. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1136987
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bi, Yunlong& Chen, Xi& Cao, Yang& Yu, Deshui& Zhao, Jia’ai& Jing, Yu…[et al.]. Nuclear Heme Oxidase-1 Inhibits Endoplasmic Reticulum Stress-Mediated Apoptosis after Spinal Cord Injury. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1136987
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136987
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر