TRPV1 Contributes to the Neuroprotective Effect of Dexmedetomidine in Pilocarpine-Induced Status Epilepticus Juvenile Rats
المؤلفون المشاركون
Tan, Xingqin
Zeng, Yong
Tu, Zhenzhen
Li, Pan
Chen, Hengsheng
Cheng, Li
Tu, Shengfen
Jiang, Li
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
To investigate the antiepileptic and neuroprotective effects of dexmedetomidine (Dex) in pilocarpine- (Pilo-) induced status epilepticus (SE) juvenile rats, rats were randomly assigned to the following six groups (n=20): normal, normal+Dex, SE, SE+Cap, SE+Dex, and SE+Dex+Cap.
The rats were treated with either diazepam (i.p., an antiepileptic drug) or Dex after the onset of SE.
The Morris water maze was used to assess rat cognitive behavior.
Flow cytometry was used to detect the concentrations of Ca2+, mitochondrial membrane potential, and reactive oxygen species.
Transmission electron microscopy was performed to evaluate specimens of brain tissue.
The levels of caspase 3 and TRPV1 were examined by western blot and immunohistochemistry (IHC).
Treatment with Dex significantly decreased the escape latency of the SE rats (P<0.05).
Capsaicin, a TRPV1 agonist, delivery aggravated the performance of SE rats.
Pathological changes in SE rat were attenuated by Dex and deteriorated by capsaicin.
Swollen mitochondria and abnormal endoplasmic reticulum were found in SE rats and were then aggravated by capsaicin and reversed by Dex.
Moreover, our data showed that Dex significantly restrained calcium overload, ROS production, and mitochondrial membrane potential loss, all of which were induced by Pilo and capsaicin (P<0.05).
Dex decreased the apoptotic rate in the Model SE group (P<0.05) and TRPV1 and caspase 3 expression in the Dex treatment group (P<0.05).
Interestingly, all these effects of Dex were partially counteracted by the TRPV1 agonist, capsaicin (P<0.05).
Our study showed that Dex exerted a neuroprotective effect in Pilo-induced SE rats by inhibiting TRPV1 expression and provided information for therapy to SE patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tan, Xingqin& Zeng, Yong& Tu, Zhenzhen& Li, Pan& Chen, Hengsheng& Cheng, Li…[et al.]. 2020. TRPV1 Contributes to the Neuroprotective Effect of Dexmedetomidine in Pilocarpine-Induced Status Epilepticus Juvenile Rats. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137052
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tan, Xingqin…[et al.]. TRPV1 Contributes to the Neuroprotective Effect of Dexmedetomidine in Pilocarpine-Induced Status Epilepticus Juvenile Rats. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1137052
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tan, Xingqin& Zeng, Yong& Tu, Zhenzhen& Li, Pan& Chen, Hengsheng& Cheng, Li…[et al.]. TRPV1 Contributes to the Neuroprotective Effect of Dexmedetomidine in Pilocarpine-Induced Status Epilepticus Juvenile Rats. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137052
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137052
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر