Protective Effect of Iridoid Glycosides of the Leaves of Syringa oblata Lindl. on Dextran Sulfate Sodium-Induced Ulcerative Colitis by Inhibition of the TLR24MyD88NF-κB Signaling Pathway

المؤلفون المشاركون

Wang, Zhongjiang
Zhang, Yifang
Han, Dandan
Yu, Shen
An, Chiying
Zhong, Haijing
Xu, Yuan
Jiang, Lianzhou
Liu, Xin

المصدر

BioMed Research International

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-04-01

دولة النشر

مصر

عدد الصفحات

13

التخصصات الرئيسية

الطب البشري

الملخص EN

Iridoid glycoside (IG) is the major active fraction extracted from the leaves of Syringa oblata Lindl.

In view of its antimicrobial and antidiarrheal potential, it could be beneficial for the treatment of ulcerative colitis (UC).

In the present study, IG (20, 40, and 80 mg/kg) was administered orally for 14 days to dextran sulfate sodium- (DSS-) induced colitis rats.

The anti-inflammatory effects of IG on DSS-induced UC were evaluated by comparing observations in DSS-induced colitis and drug-treated groups using disease activity index (DAI), macroscopic score, histological analysis, and apoptosis assay.

To elucidate the antioxidant mechanisms of IG on NOX-dependent ROS production, the activities of 8-OHdG, NOX1, and NOX2 in DSS-induced colitis were determined.

The levels of proinflammatory cytokines such as IL-2, IL-4, IL-5, IL-12p40, and IL-13 were detected.

The inflammation-associated protein and mRNA expressions of TLR-2, TLR-4, MyD88, and NF-κBp65 were assessed by immunohistochemistry and real-time quantitative PCR, respectively.

The results suggested that IG treatment significantly reduced DAI, macroscopic score, and histological damage compared to untreated animals (p<0.01), whereas administration of IG remarkably attenuated the upregulation of 8-OHdG, NOX1, and NOX2 and the expression of proinflammatory cytokines such as IL-2, IL-4, IL-5, IL-12p40, and IL-13 in DSS-treated rats in a concentration-dependent manner.

In addition, IG treatment could dose dependently suppress the protein and mRNA levels of TLR-2, TLR-4, MyD88, and NF-κBp65.

The dose of IG that produced the most significant protective effect was 80 mg/kg.

The above results demonstrate that IG exerts its inhibitory effect on cell apoptosis, oxidative stress, and proinflammatory cytokines in DSS-induced colitis through modulation of the TLR2/4/MyD88/NF-κB signaling pathway.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zhang, Yifang& Han, Dandan& Yu, Shen& An, Chiying& Liu, Xin& Zhong, Haijing…[et al.]. 2020. Protective Effect of Iridoid Glycosides of the Leaves of Syringa oblata Lindl. on Dextran Sulfate Sodium-Induced Ulcerative Colitis by Inhibition of the TLR24MyD88NF-κB Signaling Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1137116

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zhang, Yifang…[et al.]. Protective Effect of Iridoid Glycosides of the Leaves of Syringa oblata Lindl. on Dextran Sulfate Sodium-Induced Ulcerative Colitis by Inhibition of the TLR24MyD88NF-κB Signaling Pathway. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1137116

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zhang, Yifang& Han, Dandan& Yu, Shen& An, Chiying& Liu, Xin& Zhong, Haijing…[et al.]. Protective Effect of Iridoid Glycosides of the Leaves of Syringa oblata Lindl. on Dextran Sulfate Sodium-Induced Ulcerative Colitis by Inhibition of the TLR24MyD88NF-κB Signaling Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1137116

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1137116