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Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis
المؤلفون المشاركون
Chandrasekharan, N. Vishvanath
Wickramasinghe, Gammadde Hewa Ishan Maduka
Rathnayake, Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika
Weerasinghe, Mahindagoda Siril Samantha
Wijesundera, Ravindra Lakshman Chundananda
Wijesundera, Wijepurage Sandhya Sulochana
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-08-10
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
It is essential that major carbohydrate polymers in the lignocellulosic biomass are converted into fermentable sugars for the economical production of energy.
Xylan, the major component of hemicelluloses, is the second most naturally abundant carbohydrate polymer comprising 20–40% of the total biomass.
Endoxylanase (EXN) hydrolyzes xylan into mixtures of xylooligosaccharides.
The objective of this study was to genetically modify Pichia stipitis, a pentose sugar fermenting yeast species, to hydrolyze xylan into xylooligosaccharides via cloning and heterologous extracellular expression of EXNI gene from locally isolated Trichoderma virens species.
Pichia stipitis was engineered to carry the EXNI gene of T.
virens using pGAPZα expression vector.
The open reading frame encodes 191 amino acids and SDS-PAGE analysis revealed a 24 kDA recombinant protein.
The EXNI activity expressed by recombinant P.
stipitis clone under standard conditions using 1% beechwood xylan was 31.7 U/ml.
Molecular docking and molecular dynamics simulations were performed to investigate EXNI-xylan interactions.
Free EXNI and xylan bound EXNI exhibited similar stabilities and structural behavior in aqueous medium.
Furthermore, this in silico work opens avenues for the development of newer generation EXN proteins that can perform better and have enhanced catalytic activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wickramasinghe, Gammadde Hewa Ishan Maduka& Rathnayake, Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika& Chandrasekharan, N. Vishvanath& Weerasinghe, Mahindagoda Siril Samantha& Wijesundera, Ravindra Lakshman Chundananda& Wijesundera, Wijepurage Sandhya Sulochana. 2017. Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137281
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wickramasinghe, Gammadde Hewa Ishan Maduka…[et al.]. Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1137281
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wickramasinghe, Gammadde Hewa Ishan Maduka& Rathnayake, Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika& Chandrasekharan, N. Vishvanath& Weerasinghe, Mahindagoda Siril Samantha& Wijesundera, Ravindra Lakshman Chundananda& Wijesundera, Wijepurage Sandhya Sulochana. Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137281
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137281
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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