The Overexpression of Keratin 23 Promotes Migration of Ovarian Cancer via Epithelial-Mesenchymal Transition
المؤلفون المشاركون
Ren, Meng
Gao, Yan
Chen, Qi
Zhao, Hongyu
Zhao, Xiaoting
Yue, Wentao
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-02
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background.
Keratin 23 (KRT23) is a new member of the KRT gene family and known to be involved in the development and migration of various types of tumors.
However, the role of KRT23 in ovarian cancer (OC) remains unclear.
This study is aimed at investigating the function of KRT23 in OC.
Methods.
The expression of KRT23 in normal ovarian and OC tissues was determined using the Oncomine database and immunohistochemical staining.
Reverse transcription quantitative polymerase chain reaction assay was used to analyze the expression of KRT23 in normal ovarian epithelial cell lines and OC cell lines.
Small interfering RNA (siRNA), wound healing assay, and transwell assay were conducted to detect the effects of KRT23 on OC cell migration and invasion.
Further mechanistic studies were verified by the Gene Expression Profiling Interactive Analysis platform, Western blotting, and immunofluorescence staining.
Results.
KRT23 was highly expressed in OC tissues and cell lines.
High KRT23 expression could regulate OC cell migration and invasion, and the reduction of KRT23 by siRNA inhibited the migration and invasion of OC cells in vitro.
Furthermore, KRT23 mediated epithelial-mesenchymal transition (EMT) by regulating p-Smad2/3 levels in the TGF-β/Smad signaling pathway.
Conclusions.
These results demonstrate that KRT23 plays an important role in OC migration via EMT by regulating the TGF-β/Smad signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ren, Meng& Gao, Yan& Chen, Qi& Zhao, Hongyu& Zhao, Xiaoting& Yue, Wentao. 2020. The Overexpression of Keratin 23 Promotes Migration of Ovarian Cancer via Epithelial-Mesenchymal Transition. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1137362
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ren, Meng…[et al.]. The Overexpression of Keratin 23 Promotes Migration of Ovarian Cancer via Epithelial-Mesenchymal Transition. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1137362
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ren, Meng& Gao, Yan& Chen, Qi& Zhao, Hongyu& Zhao, Xiaoting& Yue, Wentao. The Overexpression of Keratin 23 Promotes Migration of Ovarian Cancer via Epithelial-Mesenchymal Transition. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1137362
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137362
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر