Effects of Cigarette Smoke Condensate on Growth and Biofilm Formation by Mycobacterium tuberculosis
المؤلفون المشاركون
Anderson, Ronald
Cholo, Moloko C.
Rasehlo, Sipho S. M.
Venter, Eudri
Venter, Chantelle
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-19
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background and Objectives.
Cigarette smoke (CS) is a major risk factor contributing to the burden of tuberculosis.
Little is known, however, about the effects of CS exposure on growth and persistence of Mycobacterium tuberculosis (Mtb) organisms.
This issue has been addressed in the current study, which is focused on the effects of cigarette smoke condensate (CSC) on the growth and viability of Mtb planktonic and biofilm-forming cultures.
Materials and Methods.
The planktonic and biofilm-forming cultures were prepared in Middlebrook 7H9 and Sauton broth media, respectively, using Mtb strain, H37Rv.
The effects of CSC at concentrations of 0.05-3.12 mg/L on growth, biofilm formation and structure were evaluated using microplate Alamar Blue assay, spectrophotometric procedure and scanning electron microscopy (SEM), respectively.
Involvement of reactive oxygen species in CSC-mediated biofilm formation was investigated by including catalase in biofilm-forming cultures.
Results.
CSC did not affect the growth of planktonic bacteria, but rather led to a statistically significant increase in biofilm formation at concentrations of 0.4-3.12 mg/L, as well as in the viability of biofilm-forming bacteria at CSC concentrations of 0.2-1.56 mg/L.
SEM confirmed an agglomerated biofilm matrix and irregular bacterial morphology in CSC-treated biofilms.
Inclusion of catalase caused significant attenuation of CSC-mediated augmentation of biofilm formation by Mtb, implying involvement of oxidative stress.
These findings demonstrate that exposure of Mtb to CSC resulted in increased biofilm formation that appeared to be mediated, at least in part, by oxidative stress, while no effect on planktonic cultures was observed.
Conclusion.
Smoking-related augmentation of biofilm formation by Mtb may contribute to persistence of the pathogen, predisposing to disease reactivation and counteracting the efficacy of antimicrobial chemotherapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cholo, Moloko C.& Rasehlo, Sipho S. M.& Venter, Eudri& Venter, Chantelle& Anderson, Ronald. 2020. Effects of Cigarette Smoke Condensate on Growth and Biofilm Formation by Mycobacterium tuberculosis. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1137366
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cholo, Moloko C.…[et al.]. Effects of Cigarette Smoke Condensate on Growth and Biofilm Formation by Mycobacterium tuberculosis. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1137366
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cholo, Moloko C.& Rasehlo, Sipho S. M.& Venter, Eudri& Venter, Chantelle& Anderson, Ronald. Effects of Cigarette Smoke Condensate on Growth and Biofilm Formation by Mycobacterium tuberculosis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1137366
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137366
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر