Identification and Characterization of MAPK Signaling Pathway Genes and Associated lncRNAs in the Ileum of Piglets Infected by Clostridium perfringens Type C
المؤلفون المشاركون
Luo, Ruirui
Huang, Xiaoyu
Yan, Zunqiang
Gao, Xiaoli
Wang, Pengfei
Yang, Qiaoli
Wang, Wei
Xie, Kaihui
Gun, Shuangbao
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-13
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Clostridium perfringens type C (C.
perfringens type C) is one of the main microbial pathogens responsible for piglet diarrhea worldwide, causing substantial economic losses for pig-rearing industries.
The mitogen-activated protein kinase (MAPK) signaling pathway is a key regulator of inflammatory bowel disease, especially necrotic enteritis.
However, whether and how the MAPK signaling pathway is involved in regulating the process of piglet diarrhea when challenged by C.
perfringens type C are still unknown.
Here, we screened 38 differentially expressed genes (DEGs) in piglets’ ileum tissues experimentally infected with C.
perfringens type C that were enriched in the Sus scrofa MAPK signaling pathway, based on our previous transcriptome data.
Of these DEGs, 12 genes (TRAF2, MAPK8, and GADD45G, among others) were upregulated whereas 26 genes (MAPK1, TP53, and CHUK, among others) were downregulated in the infected group.
Our results showed that MAPK1, TP53, MAPK8, MYC, and CHUK were in the core nodes of the PPI network.
Additionally, we obtained 35 lncRNAs from the sequencing data, which could be trans-targeted to MAPK signaling pathway genes and were differentially expressed in the ileum tissues infected with C.
perfringens.
We used qRT-PCR to verify the expression levels of genes and lncRNAs related to the MAPK signaling pathway; their expression patterns were consistent with RNA sequencing data.
Our results provide strong support for deeply exploring the role of the MAPK signaling pathway in diarrhea caused by C.
perfringens type C.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Luo, Ruirui& Huang, Xiaoyu& Yan, Zunqiang& Gao, Xiaoli& Wang, Pengfei& Yang, Qiaoli…[et al.]. 2020. Identification and Characterization of MAPK Signaling Pathway Genes and Associated lncRNAs in the Ileum of Piglets Infected by Clostridium perfringens Type C. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1137482
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Luo, Ruirui…[et al.]. Identification and Characterization of MAPK Signaling Pathway Genes and Associated lncRNAs in the Ileum of Piglets Infected by Clostridium perfringens Type C. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1137482
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Luo, Ruirui& Huang, Xiaoyu& Yan, Zunqiang& Gao, Xiaoli& Wang, Pengfei& Yang, Qiaoli…[et al.]. Identification and Characterization of MAPK Signaling Pathway Genes and Associated lncRNAs in the Ileum of Piglets Infected by Clostridium perfringens Type C. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1137482
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137482
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر