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Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by Engineered Streptomyces avermitilis
المؤلفون المشاركون
Wang, Shu-Yang
Bo, Yong-Heng
Zhou, Xiang
Chen, Ji-Hong
Li, Wen-Jian
Liang, Jian-Ping
Xiao, Guo-Qing
Wang, Yu-Chen
Liu, Jing
Hu, Wei
Jiang, Bo-Ling
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-24
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Heavy-ion irradiation technology has advantages over traditional methods of mutagenesis.
Heavy-ion irradiation improves the mutation rate, broadens the mutation spectrum, and shortens the breeding cycle.
However, few data are currently available regarding its effect on Streptomyces avermitilis morphology and productivity.
In this study, the influence of heavy-ion irradiation on S.
avermitilis when cultivated in approximately 10 L stirred-tank bioreactors was investigated.
The specific productivity of the avermectin (AVM) B1a-producing mutant S.
avermitilis 147-G58 increased notably, from 3885 to 5446 μg/mL, approximately 1.6-fold, compared to the original strain.
The mycelial morphology of the mutant fermentation processes was microscopically examined.
Additionally, protein and metabolite identification was performed by using SDS-PAGE, 2- and 3-dimensional electrophoresis (2DE and 3DE).
The results showed that negative regulation gene deletion of mutants led to metabolic process upregulating expression of protein and improving the productivity of an avermectin B1a.
The results showed that the heavy-ion beam irradiation dose that corresponded to optimal production was well over the standard dose, at approximately 80 Gy at 220 AMeV (depending on the strain).
This study provides reliable data and a feasible method for increasing AVM productivity in industrial processes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Shu-Yang& Bo, Yong-Heng& Zhou, Xiang& Chen, Ji-Hong& Li, Wen-Jian& Liang, Jian-Ping…[et al.]. 2017. Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by Engineered Streptomyces avermitilis. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1137604
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Shu-Yang…[et al.]. Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by Engineered Streptomyces avermitilis. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1137604
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Shu-Yang& Bo, Yong-Heng& Zhou, Xiang& Chen, Ji-Hong& Li, Wen-Jian& Liang, Jian-Ping…[et al.]. Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by Engineered Streptomyces avermitilis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1137604
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137604
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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