Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49
المؤلفون المشاركون
Mohamed, Hassan
Naz, Tahira
Ullah, Samee
Song, Yuanda
Mustafa, Kiren
Nazir, Yusuf
Nosheen, Shaista
Halim, Hafiy
Fazili, Abu Bakr Ahmad
Li, Shaoqi
Yang, Wu
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-29
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Carotenoids produced by microbial sources are of industrial and medicinal importance due to their antioxidant and anticancer properties.
In the current study, optimization of β-carotene production in M.
circinelloides strain 277.49 was achieved using response surface methodology (RSM).
Cerulenin and ketoconazole were used to inhibit fatty acids and the sterol biosynthesis pathway, respectively, in order to enhance β-carotene production by diverting metabolic pool towards the mevalonate pathway.
All three variables used in screening experiments were found to be significant for the production of β-carotene.
The synergistic effect of the C/N ratio, cerulenin, and ketoconazole was further evaluated and optimized for superior β-carotene production using central composite design of RSM.
Our results found that the synergistic combination of C/N ratios, cerulenin, and ketoconazole at different concentrations affected the β-carotene productions significantly.
The optimal production medium (std.
order 11) composed of C/N 25, 10 μg/mL cerulenin, and 150 mg/L ketoconazole, producing maximum β-carotene of 4.26 mg/L (0.43 mg/g) which was 157% greater in comparison to unoptimized medium (1.68 mg/L, 0.17 mg/g).
So, it was concluded that metabolic flux had been successfully redirected towards the mevalonate pathway for enhanced β-carotene production in CBS 277.49.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Naz, Tahira& Nazir, Yusuf& Nosheen, Shaista& Ullah, Samee& Halim, Hafiy& Fazili, Abu Bakr Ahmad…[et al.]. 2020. Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1137827
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Naz, Tahira…[et al.]. Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1137827
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Naz, Tahira& Nazir, Yusuf& Nosheen, Shaista& Ullah, Samee& Halim, Hafiy& Fazili, Abu Bakr Ahmad…[et al.]. Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1137827
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137827
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر