Molecular Cloning, Expression Profiling, and Marker Validation of the Chicken Myoz3 Gene

الملخص EN

Myozenin3 (Myoz3) has been reported to bind multiple Z-disc proteins and hence play a key role in signal transduction and muscle fiber type differentiation.

The purpose of current study is to better understand the basic characteristics of Myoz3.

Firstly, we cloned the ORF (open reading frame) of the Myoz3 gene.

AA (amino acid) sequence analysis revealed that the Myoz3 gene encodes a 26 kDa protein which have 97% identities with that of turkey.

Expression profiling showed that Myoz3 mRNA is mainly expressed in leg muscle and breast muscle.

Furthermore, we investigated Myoz3 gene polymorphisms in two broiler breeds, the Yellow Bantam (YB) and the Avian.

Five SNPs (single nucleotide polymorphisms) were identified in the YB breed and 3 were identified in the Avian breed.

Genotypes and haplotype were constructed and their associations with carcass traits were analyzed.

In the YB breed, c.516 C>T had a strong effect on both shank bone length and the L⁎ value of breast muscle, and the H1H3 diplotype had the highest FC compared to other diplotypes.

The markers identified in this study may serve as useful targets for the marker-assisted selection (MAS) of growth and meat quality traits in chickens.