Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells

المؤلفون المشاركون

Yamaza, Haruyoshi
Nonaka, Kazuaki
Sato, Hiroshi
Kato, Hiroki
Nguyen, Huong Thi Nguyen
Pham, Thanh Thi Mai
Han, Xu
Hirofuji, Yuta
Masuda, Keiji

المصدر

BioMed Research International

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-03-19

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الطب البشري

الملخص EN

Embryonic trisomy leads to abortion or congenital genetic disorders in humans.

The most common autosomal chromosome abnormalities are trisomy of chromosomes 13, 18, and 21.

Although alteration of gene dosage is thought to contribute to disorders caused by extra copies of chromosomes, genes associated with specific disease phenotypes remain unclear.

To generate a normal cell from a trisomic cell as a means of etiological analysis or candidate therapy for trisomy syndromes, we developed a system to eliminate a targeted chromosome from human cells.

Chromosome 21 was targeted by integration of a DNA cassette in HeLa cells that harbored three copies of chromosome 21.

The DNA cassette included two inverted loxP sites and a herpes simplex virus thymidine kinase (HSV-tk) gene.

This system causes missegregation of chromosome 21 after expression of Cre recombinase and subsequently enables the selection of cells lacking the chromosome by culturing in a medium that includes ganciclovir (GCV).

Cells harboring only two copies of chromosome 21 were efficiently induced by transfection of a Cre expression vector, indicating that this approach is useful for eliminating a targeted chromosome.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Sato, Hiroshi& Kato, Hiroki& Yamaza, Haruyoshi& Masuda, Keiji& Nguyen, Huong Thi Nguyen& Pham, Thanh Thi Mai…[et al.]. 2017. Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1137906

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Sato, Hiroshi…[et al.]. Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells. BioMed Research International No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1137906

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Sato, Hiroshi& Kato, Hiroki& Yamaza, Haruyoshi& Masuda, Keiji& Nguyen, Huong Thi Nguyen& Pham, Thanh Thi Mai…[et al.]. Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1137906

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1137906