Polysaccharides from Trichosanthes Fructus via Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology
المؤلفون المشاركون
Chen, Fujia
Li, Dahong
Shen, Hongqi
Wang, Chunhong
Li, Enzhong
Xing, Huihui
Guo, Li
Zhao, Qingchun
Shi, Junhao
Nguyen, Hoang
Liu, Jiayang
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-09-25
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
An efficient procedure for ultrasound-assisted enzymatic extraction of crude polysaccharides from Trichosanthes Fructus (crude TFP) using response surface methodology (RSM) was developed.
The Box–Behnken design was applied to optimize the effects of pH (X1), enzyme amount (X2), extraction temperature (X3), and liquid-to-solid ratio (X4) on the extraction.
The statistical analysis indicated that the independent variables (X4, X2, and X3), the quadratic coefficients (X12, X22, X32, and X42), and the interaction coefficient (X1X3) had significant impact on the yield of crude TFP.
The optimal conditions were determined as follows: pH 4.5, enzyme amount 5000 u/g, extraction temperature 45°C, and liquid-to-solid ratio 30 ml/g.
The experimental yield of crude TFP was 6.58%, which was very close to the predicted yield of 6.71%.
TFPI was then purified and characterized with Sephadex G-100 column, UV-Vis, GPC, and FT-IR.
The average molecular weight of TFPI was calculated to be 1.49 × 105 Da.
TFPI exhibited strong reducing power and possessed not only remarkable scavenging activities against ABTS•+ and DPPH radicals, but also high antitumor activities in C4-2, DU145, and PC3 cells.
The results suggest that Trichosanthes Fructus and TFPI could be a novel potent natural medicine with antioxidant and antitumor activities.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Fujia& Li, Dahong& Shen, Hongqi& Wang, Chunhong& Li, Enzhong& Xing, Huihui…[et al.]. 2017. Polysaccharides from Trichosanthes Fructus via Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1137977
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Fujia…[et al.]. Polysaccharides from Trichosanthes Fructus via Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1137977
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Fujia& Li, Dahong& Shen, Hongqi& Wang, Chunhong& Li, Enzhong& Xing, Huihui…[et al.]. Polysaccharides from Trichosanthes Fructus via Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1137977
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137977
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر