Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells

المؤلفون المشاركون

Dai, Lei
Wang, Gang
Pan, Wensheng

المصدر

BioMed Research International

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-01-18

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

To explore the mechanisms by which andrographolide inhibits gastric cancer cell proliferation and metastasis, we employed the gastric cell line SGC7901 to investigate the anticancer effects of andrographolide.

The cell survival ratio, cell migration and invasion, cell cycle, apoptosis, and matrix metalloproteinase activity were assessed.

Moreover, western blotting and real-time PCR were used to examine the protein expression levels and the mRNA expression levels, respectively.

The survival ratio of cells decreased with an increasing concentration of andrographolide in a dose-dependent manner.

Consistent results were also obtained using an apoptosis assay, as detected by flow cytometry.

The cell cycle was blocked at the G2/M2 phase by andrographolide treatment, and the proportion of cells arrested at G1/M was enhanced as the dose increased.

Similarly, wound healing and Transwell assays showed reduced migration and invasion of the gastric cancer cells at various concentrations of andrographolide.

Andrographolide can inhibit cell proliferation, invasion, and migration, block the cell cycle, and promote apoptosis in SGC7901 cells.

The mechanisms may include upregulated expression of Timp-1/2, cyclin B1, p-Cdc2, Bax, and Bik and downregulated expression of MMP-2/9 and antiapoptosis protein Bcl-2.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Dai, Lei& Wang, Gang& Pan, Wensheng. 2017. Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138000

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Dai, Lei…[et al.]. Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1138000

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Dai, Lei& Wang, Gang& Pan, Wensheng. Andrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138000

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1138000