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Bioinformatical Analysis of miRNA-mRNA Interaction Network Underlying Macrophage Aging and Cholesterol-Responsive Difference between Young and Aged Macrophages
المؤلفون المشاركون
Li, Jianqing
Yin, Xue
Zhang, Bingyu
Li, Chen
Lu, Pei-Rong
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-13
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Purpose.
Macrophage aging is involved with the occurrence and progression of age-related macular degeneration (AMD).
The purpose of this study was to identify the specific microRNAs (miRNA), mRNAs, and their interactions underlying macrophage aging and response to cholesterol through bioinformatical analysis in order to get a better understanding of the mechanism of AMD.
Methods.
The microarray data were obtained from Gene Expression Omnibus (accession GSE111304 and GSE111382).
The age-related differentially expressed genes in macrophages were identified using R software.
Further miRNA-mRNA interactions were analyzed through miRWalk, mirTarBase, starBase, and then produced by Cytoscape.
The functional annotations including Gene Ontology and KEGG pathways of the miRNA target genes were performed by the DAVID and the STRING database.
In addition, protein-protein interaction network was constructed to identify the key genes in response to exogenous cholesterol.
Results.
When comparing aged and young macrophages, a total of 14 miRNAs and 101 mRNAs were detected as differentially expressed.
Besides, 19 validated and 544 predicted miRNA-mRNA interactions were detected.
Lipid metabolic process was found to be associated with macrophage aging through functional annotations of the miRNA targets.
After being treated with oxidized and acetylated low-density lipoprotein, miR-714 and 16 mRNAs differentially expressed in response to both kinds of cholesterol between aged and young macrophages.
Among them, 6 miRNA-mRNA predicted pairs were detected.
The functional annotations were mainly related to lipid metabolism process and farnesyl diphosphate farnesyl transferase 1 (FDFT1) was identified to be the key gene in the difference of response to cholesterol between aged and young macrophages.
Conclusions.
Lipid metabolic process was critical in both macrophage aging and response to cholesterol thus was regarded to be associated with the occurrence and progression of AMD.
Moreover, miR-714-FDFT1 may modulate cholesterol homeostasis in aged macrophages and have the potential to be a novel therapeutic target for AMD.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Jianqing& Yin, Xue& Zhang, Bingyu& Li, Chen& Lu, Pei-Rong. 2020. Bioinformatical Analysis of miRNA-mRNA Interaction Network Underlying Macrophage Aging and Cholesterol-Responsive Difference between Young and Aged Macrophages. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1138046
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Jianqing…[et al.]. Bioinformatical Analysis of miRNA-mRNA Interaction Network Underlying Macrophage Aging and Cholesterol-Responsive Difference between Young and Aged Macrophages. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1138046
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Jianqing& Yin, Xue& Zhang, Bingyu& Li, Chen& Lu, Pei-Rong. Bioinformatical Analysis of miRNA-mRNA Interaction Network Underlying Macrophage Aging and Cholesterol-Responsive Difference between Young and Aged Macrophages. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1138046
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138046
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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