Downregulation of CFTR Is Involved in the Formation of Hypertrophic Scars
المؤلفون المشاركون
Liang, Xiao
Zhou, Yiwen
Du, Hengyu
Suo, Yingjun
Li, Qingfeng
Huang, Xiaolu
Zhao, Yixuan
Chen, Hao
Li, Haizhou
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Hypertrophic Scars (HTSs) are a complex fibroproliferative disorder, and their exact mechanism is still not fully understood.
In this study, we first found that cystic fibrosis transmembrane conductance regulator (CFTR) expression was downregulated in human hypertrophic scars at the RNA and protein levels by microarray data analysis, RT-PCR, and immunofluorescence (IF) staining.
To validate that this downregulation of CFTR is involved in the formation of HTSs, we then applied a mechanical overloading intervention in both wild type and CFTR-mutant mice (ΔF508).
Our results showed thatΔF508 mice exhibited delayed wound healing and a significantly larger HTS on day 28.
Masson staining revealed that there was more collagen deposition in the HTS, and Sirius red staining and IF staining showed a higher ratio of collagen 1/collagen 3 (Col1/Col3) in ΔF508 mice.
Real-time RT-PCR showed that the proinflammatory markers were higher in ΔF508 mice in all phases of scar formation, whereas the proliferation marker was similar.
Moreover, we harvested the fibroblasts from both mice.
Western blotting showed that the expression of Col1 was the same in both mice, and the expression of Col3 was significantly lower in ΔF508 mice.
However, in a mechanical overloading condition, the expression of Col1 was significantly higher in ΔF508 mice, and the expression of Col3 was the same in both mice.
Taken together, our results indicate that the downregulation of CFTR might affect the function of fibroblasts, resulting in a lower level of collagen type 3 and a higher ratio of Col1/Col3, and thus aggravate the formation of HTSs in mechanical overloading conditions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Yiwen& Zhao, Yixuan& Du, Hengyu& Suo, Yingjun& Chen, Hao& Li, Haizhou…[et al.]. 2020. Downregulation of CFTR Is Involved in the Formation of Hypertrophic Scars. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1138157
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Yiwen…[et al.]. Downregulation of CFTR Is Involved in the Formation of Hypertrophic Scars. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1138157
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Yiwen& Zhao, Yixuan& Du, Hengyu& Suo, Yingjun& Chen, Hao& Li, Haizhou…[et al.]. Downregulation of CFTR Is Involved in the Formation of Hypertrophic Scars. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1138157
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138157
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر