PEDF Is Associated with the Termination of Chondrocyte Phenotype and Catabolism of Cartilage Tissue
المؤلفون المشاركون
Ekici, Arif B.
Klinger, P.
Lukassen, S.
Ferrazzi, F.
Hotfiel, T.
Swoboda, B.
Aigner, T.
Gelse, K.
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-16
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the expression and target genes of pigment epithelium-derived factor (PEDF) in cartilage and chondrocytes, respectively.
Methods.
We analyzed the expression pattern of PEDF in different human cartilaginous tissues including articular cartilage, osteophytic cartilage, and fetal epiphyseal and growth plate cartilage, by immunohistochemistry and quantitative real-time (qRT) PCR.
Transcriptome analysis after stimulation of human articular chondrocytes with rhPEDF was performed by RNA sequencing (RNA-Seq) and confirmed by qRT-PCR.
Results.
Immunohistochemically, PEDF could be detected in transient cartilaginous tissue that is prone to undergo endochondral ossification, including epiphyseal cartilage, growth plate cartilage, and osteophytic cartilage.
In contrast, PEDF was hardly detected in healthy articular cartilage and in the superficial zone of epiphyses, regions that are characterized by a permanent stable chondrocyte phenotype.
RNA-Seq analysis and qRT-PCR demonstrated that rhPEDF significantly induced the expression of a number of matrix-degrading factors including SAA1, MMP1, MMP3, and MMP13.
Simultaneously, a number of cartilage-specific genes including COL2A1, COL9A2, COMP, and LECT were among the most significantly downregulated genes.
Conclusions.
PEDF represents a marker for transient cartilage during all neonatal and postnatal developmental stages and promotes the termination of cartilage tissue by upregulation of matrix-degrading factors and downregulation of cartilage-specific genes.
These data provide the basis for novel strategies to stabilize the phenotype of articular cartilage and prevent its degradation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Klinger, P.& Lukassen, S.& Ferrazzi, F.& Ekici, Arif B.& Hotfiel, T.& Swoboda, B.…[et al.]. 2017. PEDF Is Associated with the Termination of Chondrocyte Phenotype and Catabolism of Cartilage Tissue. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1138432
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Klinger, P.…[et al.]. PEDF Is Associated with the Termination of Chondrocyte Phenotype and Catabolism of Cartilage Tissue. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1138432
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Klinger, P.& Lukassen, S.& Ferrazzi, F.& Ekici, Arif B.& Hotfiel, T.& Swoboda, B.…[et al.]. PEDF Is Associated with the Termination of Chondrocyte Phenotype and Catabolism of Cartilage Tissue. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1138432
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138432
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر