Long Noncoding RNA SOX2-OT Exacerbates Hypoxia-Induced Cardiomyocytes Injury by Regulating miR-27a-3pTGFβR1 Axis
المؤلفون المشاركون
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-23
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
Myocardial infarction (MI) was a severe cardiovascular disease resulted from acute, persistent hypoxia, or ischemia condition.
Additionally, MI generally led to heart failure, even sudden death.
A multitude of research studies proposed that long noncoding RNAs (lncRNAs) frequently participated in the regulation of heart diseases.
The specific function and molecular mechanism of SOX2-OT in MI remained unclear.
Aim of the Study.
The current research was aimed to explore the role of SOX2-OT in MI.
Methods.
Bioinformatics analysis (DIANA tools and Targetscan) and a wide range of experiments (CCK-8, flow cytometry, RT-qPCR, luciferase reporter, RIP, caspase-3 activity, trans-well, and western blot assays) were adopted to investigate the function and mechanism of SOX2-OT.
Results.
We discovered that hypoxia treatment decreased cell viability but increased cell apoptosis.
Besides, lncRNA SOX2-OT expression was upregulated in hypoxic HCMs.
Hereafter, we confirmed that SOX2-OT could negatively regulate miR-27a-3p levels by directly binding with miR-27a-3p, and miR-27a-3p also could negatively regulate SOX2-OT levels.
Furthermore, knockdown of SOX2-OT promoted cell proliferation, migration, and invasion, but limited cell apoptosis.
However, these effects were reversed by anti-miR-27a-5p.
Besides, we verified that miR-27a-3p binding with the 3′UTR of TGFBR1 and SOX2-OT regulated TGFβR1 level by collaborating with miR-27a-3p in HCMs.
Eventually, rescue assays validated that the influence of SOX2-OT silence or miR-27a-3p overexpression on cellular processes in cardiomyocytes injury was counteracted by TGFBR1 overexpression.
Conclusions.
Long noncoding RNA SOX2-OT exacerbated hypoxia-induced cardiomyocytes injury by regulating miR-27a-3p/TGFβR1 axis, which may provide a novel insight for heart failure treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Guang& Lin, Chunsheng. 2020. Long Noncoding RNA SOX2-OT Exacerbates Hypoxia-Induced Cardiomyocytes Injury by Regulating miR-27a-3pTGFβR1 Axis. Cardiovascular Therapeutics،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1138578
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Guang& Lin, Chunsheng. Long Noncoding RNA SOX2-OT Exacerbates Hypoxia-Induced Cardiomyocytes Injury by Regulating miR-27a-3pTGFβR1 Axis. Cardiovascular Therapeutics No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1138578
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Guang& Lin, Chunsheng. Long Noncoding RNA SOX2-OT Exacerbates Hypoxia-Induced Cardiomyocytes Injury by Regulating miR-27a-3pTGFβR1 Axis. Cardiovascular Therapeutics. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1138578
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138578
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر