Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway
المؤلفون المشاركون
Zhao, Jianning
Bao, Ni-Rong
Meng, Jia
Qian, Hong
Sun, Wenshuang
Wang, Zhenheng
Yuan, Tao
Chen, Wenxiang
Tong, Jian
Xie, Yu
Zhang, Ya
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-10-22
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Proanthocyanidins (PCs) have shown inhibition of oxidative damage by improving Nrf-2 expression in many tissues.
However, the cytoprotective effects of PCs on H2O2-induced tendon damage have not been verified.
The current study was aimed at assessing the cytoprotection of PCs on the oxidative cellular toxicity of tendon-derived stem cells (TDSCs) induced by H2O2.
The TDSCs were isolated from patellar tendons of Sprague Dawley (SD) rats, and the cells after third passage were used for subsequent experiments.
The isolated cells were identified by flow cytometry assay and multidifferentiation potential assay.
Cell Counting Kit-8 assay was performed to examine cell viability.
Real-Time PCR and Western Blot were employed to, respectively, assess the mRNA and protein expressions of Nrf-2, GCLM, NQO-1, and HO-1.
PCs significantly improved the cell viability of TDSCs.
Furthermore, H2O2 upregulated Nrf-2, GCLM, NQO-1, and HO-1 without significant difference, while the proteins expressions were increased with significant difference in PCs group and PCs + H2O2 cotreated group.
All the findings indicated that PCs could protect against the oxidative damage induced by H2O2 in TDSCs, and the cytoprotective effects might be due to the ability of PCs to activate the expressions of GCLM, HO-1, and NQO-1 via upregulating Nrf-2 signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Wenshuang& Meng, Jia& Wang, Zhenheng& Yuan, Tao& Qian, Hong& Chen, Wenxiang…[et al.]. 2017. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138654
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Wenshuang…[et al.]. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1138654
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Wenshuang& Meng, Jia& Wang, Zhenheng& Yuan, Tao& Qian, Hong& Chen, Wenxiang…[et al.]. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138654
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138654
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر