Exogenous Expressions of FTO Wild-Type and R316Q Mutant Proteins Caused an Increase in HNRPK Levels in 3T3-L1 Cells as Demonstrated by DIGE Analysis
المؤلفون المشاركون
Guzel, Nil
Kanli, Aylin
Gulkac, M. Dogan
Karaosmanoglu, Kubra
Akpinar, Gurler
Kasap, Murat
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-05-07
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Fat mass and obesity-associated protein is an enzyme that oxidatively demethylates DNA.
Although there are numerous studies regarding the catalytic function of FTO, the overall existence or absence of FTO on cellular proteome has not been investigated.
This study investigated the changes in the soluble proteome of 3T3-L1 cells upon expression of the WT and the mutant (R316Q) FTO proteins.
Protein extracts prepared from 3T3-L1 cells expressing either the WT or the mutant FTO proteins were used in DIGE experiments.
Analysis of the data revealed the number of spots matched to every member and there were 350 ± 20 spots with 30.5% overall mean coefficient of variation.
Eleven regulated protein spots were excised from the gels and identified by MALDI-TOF/TOF.
One of the identified proteins was heterogeneous nuclear ribonucleoprotein K, which displayed more than 2.6- and 3.7-fold increases in its abundance in the WT and the mutant FTO expressing cells, respectively.
Western blot analysis validated these observations.
This is the first study revealing the presence of a parallel increase in expressions of FTO and HNRNPK proteins.
This increase may codictate the metabolic changes occurring in the cell and may attribute a significance to HNRNPK in FTO-associated transformations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guzel, Nil& Kasap, Murat& Kanli, Aylin& Akpinar, Gurler& Gulkac, M. Dogan& Karaosmanoglu, Kubra. 2017. Exogenous Expressions of FTO Wild-Type and R316Q Mutant Proteins Caused an Increase in HNRPK Levels in 3T3-L1 Cells as Demonstrated by DIGE Analysis. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1138975
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guzel, Nil…[et al.]. Exogenous Expressions of FTO Wild-Type and R316Q Mutant Proteins Caused an Increase in HNRPK Levels in 3T3-L1 Cells as Demonstrated by DIGE Analysis. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1138975
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guzel, Nil& Kasap, Murat& Kanli, Aylin& Akpinar, Gurler& Gulkac, M. Dogan& Karaosmanoglu, Kubra. Exogenous Expressions of FTO Wild-Type and R316Q Mutant Proteins Caused an Increase in HNRPK Levels in 3T3-L1 Cells as Demonstrated by DIGE Analysis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1138975
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138975
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر