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Analytical Validation of a New Enzymatic and Automatable Method for d-Xylose Measurement in Human Urine Samples
المؤلفون المشاركون
Fernández-Mayoralas, Alfonso
Sánchez-Moreno, Israel
Monsalve-Hernando, Carmen
Godino, Ana
Illa, Luis
Gaspar, María Jesús
Muñoz, Guillermo Manuel
Díaz, Ana
Martín, José Luis
García-Junceda, Eduardo
Hermida, Carmen
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-09-24
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Hypolactasia, or intestinal lactase deficiency, affects more than half of the world population.
Currently, xylose quantification in urine after gaxilose oral administration for the noninvasive diagnosis of hypolactasia is performed with the hand-operated nonautomatable phloroglucinol reaction.
This work demonstrates that a new enzymatic xylose quantification method, based on the activity of xylose dehydrogenase from Caulobacter crescentus, represents an excellent alternative to the manual phloroglucinol reaction.
The new method is automatable and facilitates the use of the gaxilose test for hypolactasia diagnosis in the clinical practice.
The analytical validation of the new technique was performed in three different autoanalyzers, using buffer or urine samples spiked with different xylose concentrations.
For the comparison between the phloroglucinol and the enzymatic assays, 224 urine samples of patients to whom the gaxilose test had been prescribed were assayed by both methods.
A mean bias of −16.08 mg of xylose was observed when comparing the results obtained by both techniques.
After adjusting the cut-off of the enzymatic method to 19.18 mg of xylose, the Kappa coefficient was found to be 0.9531, indicating an excellent level of agreement between both analytical procedures.
This new assay represents the first automatable enzymatic technique validated for xylose quantification in urine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sánchez-Moreno, Israel& Monsalve-Hernando, Carmen& Godino, Ana& Illa, Luis& Gaspar, María Jesús& Muñoz, Guillermo Manuel…[et al.]. 2017. Analytical Validation of a New Enzymatic and Automatable Method for d-Xylose Measurement in Human Urine Samples. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139085
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sánchez-Moreno, Israel…[et al.]. Analytical Validation of a New Enzymatic and Automatable Method for d-Xylose Measurement in Human Urine Samples. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139085
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sánchez-Moreno, Israel& Monsalve-Hernando, Carmen& Godino, Ana& Illa, Luis& Gaspar, María Jesús& Muñoz, Guillermo Manuel…[et al.]. Analytical Validation of a New Enzymatic and Automatable Method for d-Xylose Measurement in Human Urine Samples. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139085
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139085
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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