Phenotypic and Molecular Characterization of β-Lactamases among Enterobacterial Uropathogens in Southeastern Nigeria

المؤلفون المشاركون

Shariff, Malini
Ugwu, Malachy C.
Okezie, Ugochukwu
Nnajide, C.M.
Beri, K
Iroha, I. R.
Esimone, Charles Okechukwu

المصدر

Canadian Journal of Infectious Diseases and Medical Microbiology

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-02-25

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الأحياء

الملخص EN

Little is known about the molecular basis of antibiotic resistance among uropathogens in Southeast Nigeria.

The aim of the study was to characterize enterobacterial uropathogens with respect to drug resistance.

One hundred (100) enterobacterial uropathogens were studied.

Their antibiotic susceptibility patterns were evaluated using disk diffusion, screened, and confirmed phenotypically for the presence of β-lactamases: ESBL, AmpC, carbapenemase, and MBLs.

Screen positives were further tested for various β-lactamase genes by PCR.

Our isolates showed variable resistance to most drugs tested.

Out of the 58 ESBL screen positive E.

coli, 35 were confirmed positive with PCR.

The predominant ESBL gene was blaTEM while blaSPM was the most prevalent among MBL genes.

Forty-six percentage of the screen positive Salmonella isolates coharbored blaTEM + SHV genes.

Nine of the 10 ESBL screen positive K.

pneumoniae were phenotypically and PCR positive.

Three isolates of K.

pneumoniae were positive for MBL genes.

All the 10 C.

freundii were positive for ESBL genes.

The study showed high prevalence of drug-resistant genes among the enterobacterial uropathogens.

Majority of the uropathogens harbored >1 antibiotic-resistant gene, and the most predominant gene was ESBL (blaTEM) followed by the MBL (SPM) gene.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Ugwu, Malachy C.& Shariff, Malini& Nnajide, C.M.& Beri, K& Okezie, Ugochukwu& Iroha, I. R.…[et al.]. 2020. Phenotypic and Molecular Characterization of β-Lactamases among Enterobacterial Uropathogens in Southeastern Nigeria. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139150

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Ugwu, Malachy C.…[et al.]. Phenotypic and Molecular Characterization of β-Lactamases among Enterobacterial Uropathogens in Southeastern Nigeria. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1139150

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Ugwu, Malachy C.& Shariff, Malini& Nnajide, C.M.& Beri, K& Okezie, Ugochukwu& Iroha, I. R.…[et al.]. Phenotypic and Molecular Characterization of β-Lactamases among Enterobacterial Uropathogens in Southeastern Nigeria. Canadian Journal of Infectious Diseases and Medical Microbiology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139150

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1139150