Improved High-Throughput Sequencing of the Human Oral Microbiome: From Illumina to PacBio
المؤلفون المشاركون
Zhang, Jie
Su, Lingkai
Wang, Yuan
Deng, Shuli
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-11
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background.
A comprehensive understanding of the commensal microflora and its relation to health is essential for preventing and combating diseases.
The aim of this study was to examine the structure of the oral microbiome by using different sequencing technologies.
Material and Methods.
Five preschool children with no symptoms of oral and systemic diseases were recruited.
Samples of saliva were collected.
A 468 bp insert size library was constructed on the MiSeq platform and then subjected to 300 bp paired-end sequencing.
Libraries with longer insert sizes, including a full-length 16S rDNA gene, were sequenced on the PacBio RS II platform.
Results.
A total of 122.6 Mb of raw data, including 244,967 high-quality sequences, were generated by the MiSeq platform, while 134.6 Mb of raw data, including 70,030 high-quality reads, were generated by the PacBio RS II platform.
Clustering of the unique sequences into OTUs at 3% dissimilarity resulted in an average of 225 OTUs on the MiSeq platform; however, the number of OTUs generated on the PacBio RS II platform was 449, far greater than the number of OTUs generated on the MiSeq platform.
A total of 437 species belonging to 10 phyla and 60 genera were detected by the PacBio RS II platform, while 163 species belonging to 12 phyla and 72 genera were detected by the MiSeq platform.
Conclusions.
The oral microflora of healthy Chinese children were analyzed.
Compared with traditional 16S rRNA sequencing technology, the PacBio system, despite providing a lower amount of clean data, surpassed the resolution of the MiSeq platform by improving the read length and annotating the nucleotide sequences at the species or strain level.
This trial is registered with NCT02341352.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Jie& Su, Lingkai& Wang, Yuan& Deng, Shuli. 2020. Improved High-Throughput Sequencing of the Human Oral Microbiome: From Illumina to PacBio. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1139158
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Jie…[et al.]. Improved High-Throughput Sequencing of the Human Oral Microbiome: From Illumina to PacBio. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1139158
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Jie& Su, Lingkai& Wang, Yuan& Deng, Shuli. Improved High-Throughput Sequencing of the Human Oral Microbiome: From Illumina to PacBio. Canadian Journal of Infectious Diseases and Medical Microbiology. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1139158
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139158
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر