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Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea
المؤلفون المشاركون
Xiao, Yanyan
Liu, Yong
Qin, Xiaosong
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-22
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Objectives.
The aim of this study was to evaluate the clinical application of three methods for detecting Clostridium difficile in fecal samples.
Methods.
One hundred and fifty fecal specimens were collected and tested for C.
difficile using three methods: (1) the toxigenic culture (TC); (2) the VIDAS enzyme immunoassay (EIA): the VIDAS glutamate dehydrogenase (GDH) assay and toxin A/B assay were used to detect GDH antigen and A/B toxin; and (3) the GeneXpert PCR assay.
The toxigenic culture was used as a reference to evaluate the performance of the VIDAS EIA and the GeneXpert PCR assay.
Results.
Of 150 specimens, 26 carried both A and B toxin genes, and none of the samples were positive for the binary toxin gene.
Toxin-producing C.
difficile was found in 17.3% (26/150) of the samples.
Thirty-seven GDH-positive samples were detected using the VIDAS GDH assay, and 15 toxin-positive samples were detected using the VIDAS toxin A/B assay.
The GeneXpert PCR assay was used to detect C.
difficile in 79 specimens simultaneously, and a total of 18 positive specimens were detected.
Conclusion.
The VIDAS GDH assay is useful for initial screening of C.
difficile.
The GeneXpert PCR assay is a simple and quick method.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xiao, Yanyan& Liu, Yong& Qin, Xiaosong. 2020. Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1139187
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xiao, Yanyan…[et al.]. Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1139187
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xiao, Yanyan& Liu, Yong& Qin, Xiaosong. Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea. Canadian Journal of Infectious Diseases and Medical Microbiology. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1139187
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139187
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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