Development and Evaluation of an iiPCR Assay for Salmonella and Shigella Detection on a Field-Deployable PCR System
المؤلفون المشاركون
Du, Tian
Lin, Ji-hong
Zhao, Jun-hua
Wang, Hai-bo
Mo, Qiu-hua
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-07
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Background.
Salmonella and Shigella are often associated with fecal-oral transmission and cause large-scale outbreaks in centralized catering units and, therefore, should be frequently and strictly monitored, especially among food handlers.
However, no specific and sensitive on-site detection method is available until now.
Methods.
In this study, an insulated isothermal PCR assay for the detection of Salmonella and Shigella on a field-deployable PCR system was developed.
Specificity, sensitivity, reproducibility, and clinical accuracy of the assay were characterized and evaluated.
Results.
The insulated isothermal PCR assay could be completed within 58 minutes with minimal pretreatment needed.
The assay was specific and with good reproducibility.
The limit of detection was 103 CFU/mL and 101 CFU/mL for Salmonella and Shigella, respectively, which was comparable to multiplex real-time PCR.
Mock on-site clinical evaluation results showed that the analytical sensitivity and specificity of the insulated isothermal PCR assay were 100% and 96.6%, while the positive predictive value and negative predictive value were 94.1% and 100%, respectively.
Conclusion.
Based on our results, we believe that the assay developed herein could serve as an alternative method for preliminary screening and provide a valuable platform for the on-site detection of Salmonella and Shigella, especially in resource-limited and developing countries.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Du, Tian& Lin, Ji-hong& Zhao, Jun-hua& Wang, Hai-bo& Mo, Qiu-hua. 2020. Development and Evaluation of an iiPCR Assay for Salmonella and Shigella Detection on a Field-Deployable PCR System. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1139214
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Du, Tian…[et al.]. Development and Evaluation of an iiPCR Assay for Salmonella and Shigella Detection on a Field-Deployable PCR System. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1139214
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Du, Tian& Lin, Ji-hong& Zhao, Jun-hua& Wang, Hai-bo& Mo, Qiu-hua. Development and Evaluation of an iiPCR Assay for Salmonella and Shigella Detection on a Field-Deployable PCR System. Canadian Journal of Infectious Diseases and Medical Microbiology. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1139214
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139214
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر