Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications
المؤلفون المشاركون
Simair, Altaf Ahmed
Qureshi, Abdul Sattar
Khushk, Imrana
Ali, Chaudhry Haider
Lashari, Safia
Bhutto, Muhammad Aqeel
Mangrio, Ghulam Sughra
Lu, Changrui
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-12
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Amylase is an industrially important enzyme and applied in many industrial processes such as saccharification of starchy materials, food, pharmaceutical, detergent, and textile industries.
This research work deals with the optimization of fermentation conditions for α-amylase production from thermophilic bacterial strain Bacillus sp.
BCC 01-50 and characterization of crude amylase.
The time profile of bacterial growth and amylase production was investigated in synthetic medium and maximum enzyme titer was observed after 60 h.
In addition, effects of different carbon sources were tested as a substrate for amylase production and molasses was found to be the best.
Various organic and inorganic compounds, potassium nitrate, ammonium chloride, sodium nitrate, urea, yeast extract, tryptone, beef extract, and peptone, were used and beef extract was found to be the best among the nitrogen sources used.
Temperature, pH, agitation speed, and size of inoculum were also optimized.
Highest enzyme activity was obtained when the strain was cultured in molasses medium for 60 h in shaking incubator (150 rpm) at 50°C and pH 8.
Crude amylase showed maximal activity at pH 9 and 65°C.
Enzyme remained stable in alkaline pH range 9-10 and 60–70°C.
Crude amylase showed great potential for its application in detergent industry and saccharification of starchy materials.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Simair, Altaf Ahmed& Qureshi, Abdul Sattar& Khushk, Imrana& Ali, Chaudhry Haider& Lashari, Safia& Bhutto, Muhammad Aqeel…[et al.]. 2017. Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139387
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Simair, Altaf Ahmed…[et al.]. Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139387
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Simair, Altaf Ahmed& Qureshi, Abdul Sattar& Khushk, Imrana& Ali, Chaudhry Haider& Lashari, Safia& Bhutto, Muhammad Aqeel…[et al.]. Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp. BCC 01-50 and Potential Applications. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139387
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139387
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر